They Call It Heresy,
We Call It Genuine Science
We designed the genes' primers,
Ordered them along the oligomers.
Our aim is an elaborate one,
It involves molecular cloning,
Sequence characterization, and
Relative expression analysis of
Bovine Trefoil Factors.
Now we hope to clone the gene,
The gene which is of a bovine origin,
By extensive working hours input,
And bearing in mind the risks,
Of not getting the desired output,
The possibility of failure always therein,
But pregnancy, healing & immunity it's governing.
Three types of trefoil factors there are,
TFF1: It suppresses gastric carcinoma,
And also helps in pregnancy,
TFF2: Helps exclusively in cancer research,
TFF3: Helps exclusively in pregnancy maintenance,
And also our prime interest.
After cloning the genes,
We have to sequence them,
And after characterization,
We have to analyse them,
After relative expression.
My M.Tech dissertation research topic is molecular cloning, sequence characterization, and relative expression analysis of Bovine Trefoil Factors and we will be working with water buffalo species.
I completed this work under the guidance of Dr AK Mohanty with additional working guidance from my dear elder sisterly lab mate Dr S G Chaudhary neé Rana.
The complete 2nd year was a research year.
HP Poem #1306
©Atul Kaushal
