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Àŧùl Dec 2016
Preventing contamination,
A constant challenge in cell culture.
Contamination not only affects,
The culture in question and,
Costs time and money,
But also endangers the reproducibility of results.

No cell culture problem,
Is as universal as that of culture loss
Due to contamination.

Generally, contamination may be separated,
Into categories of microbial,
And eukaryotic contamination.

Examples of microbial contamination include:
Bacteria (including Mycoplasma),
Fungi and yeast;
Eukaryotic contamination includes:
Cross-contamination with other cell lines.

Bacteria, yeast and fungi,
The three more common types of contamination,
But luckily these forms are often detectable,
Under the microscope and,
By visual cues,
Like colour or turbidity changes in the medium.

Mycoplasma is a small genus of bacteria,
That lack a cell wall and for this reason,
They remain unaffected by common antibiotics.
They are also difficult to detect,
With standard microscopes,
Due to their size, about 0.1 μm in diameter,
And the fact that they often attach to host cells.


To prevent contamination,
Use 70% ethanol for disinfecting,
Equipment & surfaces,
Related to cell culture.
Sterile filter the media first,
Before bringing to the lab.

Fetal Bovine Serum,
A potential source of contamination,
Contains mycoplasma.
Filter it at 0.1 μm, or,
Gamma irradiate it.
Aseptic technique,
Necessary.

The laboratory workers be the last,
But not the least source of contamination.
Teach them the ideal laboratory practices,
To ensure asepticity in a laboratory.
Source: American Laboratory

For revising an important topic from Animal Cell Culture.

HP Poem #1299
©Atul Kaushal
King Panda Jul 2017
a haw and saw.
a thorn.

fruit: it is ecstasy
never bit and
undeniable.

you slurp—a cat licking
its paws
ruby and clear.
moth and cloud
drape over fruit,
make up sparkling nectar.

love is sickening.
you spend five dollars on
a rose at a bar for
a girl you will never see
again.

she will take the flower and
throw it in the trash
outside with the hundreds of
other roses.

no matter.
they have fruit, and fruit
concludes. it is life
cut with claws.
their beauty, seemingly to
be always in the clusters above.

*******, rose. **** your dew.

they seem to say. that’s when
the light hits and microbial
bleeds to miss ruby.

JAZZ!

at night retrains
beauty, makes it edible.
the rose, changing the color
of its dew—black pearl in
this drape of mystery-shaped
night.
Anonymous May 2014
"And now please welcome today's anti-terrorism speaker, Anonymous!"

[anonymous applause, dwindling out]

"Thanks, everyone. The reason I prefer anonymity should be self-evident, but just to make it clear, I wish to avoid the recrimination of the hostile element."

"Before I got here I was just reading, and believe me I'm still not believing, but it would seem, on the whole, that planetary aggression is on the slow."

A hand is raised
A hand is ignored
The speaker moistens his lips
Prepared to emit a bit more.

"I have stats and stories
Tortuous anecdotes about little girls and boys
Food and sanitation is a crime itself
And I'm prepared to say we live in our own hell."

Arms upheld wither down
As new hands reach for attention
But the speaker ignores them all
Intent on his own presentation.

"The reason for hate
Is more or less clear
We fiercely believe one thing
As they devoutly believe another.

But do not fear!
We are right and they are wrong
They saddle their own children with a death song
No cartoons of basic morality
Just legs with bombs
Made to go off remotely."

An angry rustle
Amidst lowered hands
Quieting now
Like they're getting the hang of it.

"Humans are robots
Programmable, malleable and sometimes trustworthy
Highly complicated machinery!
Indoctrination is the virus
That seeks to destroy the outside."

Again the raised hands
And eyebrows too
All these fluttering robots
Fluttering in a pseudo-free zoo.

Ignoring the obvious
The speaker plods onwards
But modulates his voice
Against their trained reactions.

"We need to accept and enfold
An ideology only thousands of years old
To mutate and twist
Into what our children might wish."

Someone yells "Disney!"
Another mutters "Black whiteys"
But there are a few
Who remain to hear it through.

"Despite what you think
Despite who you are
Against all you've been taught
We've come quite far.

You may not know your son
You may not know your daughter
But leave them alone
And tomorrow may happen.

Put the guns aside
Drink from your hidden bottles without shame
You are who you are
And you should let them be them."

This is not what anyone wanted
Anyone over the age of ten
This is not what anyone wanted
With children and the urge to brainwash them.

The room trickles out
Leaving the most devout
Devoted to the future
Any future left standing.

But amidst this group
Are hard-liner elements
And one has a voice
Cutting through it all
To ask, "What about bomber babies?"

And riding right on top
Is a fat slobbery Republican fop
Demanding in his self-entitled way
"What the **** about America?"

The speaker shrugs
As if to indicate
Which question
Is more stupid.

"We seek to leave the planet
And develop tech to make it happen
You go your way
And we go ours."

The room is smaller now
They indulge in eye contact
Personal communications
Words, hands, heads and eyebrows.

The speaker sighs
As if on the cusp of absolute honesty
Then spills his true guts
To these few radicals and emissaries:

"Our worst enemy is ourselves
Through millennia fashioning our own hells
Subjugation of non-prominent DNA
Believing destruction will pave the way.

But on a not-much larger scale
We're just cheap entertainment
For every other race
That crawled up this hill."

The crowd is slightly subdued
Probably more from shame
Than anything
Because shame is in the DNA
And experienced by everyone.

But we can always rely
On some fat Republican to decry
"But not me!
And for sure not my children!"

And now even more file out
Hearts emptied and minds afloat
Now it's just the sweating speaker
And a few odd haters.

"We're a microbial phenomenon
Miraculously still alive
And still inept
At staying alive."

He waves a casual hand like a maestro
And behind him the stage glows
A 30x30 screen descends
Illuminating bugs as they crawl.

"We're slightly smarter
But no more hardier
Than Hymenoptera
Except we can leave this planet."

Red-faced and obviously insulted
The old fat plushy storms out
Leaving now just a few
To adopt this future-flung view.

"We need to terraform and colonize
Sure, and design space suits
Pleasing to the eye
But ultimately,
We need to get the hell gone."

One clap, one frown
The speaker shrugs
As if wondering
Why aren't we all gone?

And so he is left
With the clean-up crew
And one fruitcake
Who asks
"Will God come with us?"
K Balachandran Jan 2016
1.
On a nameless planet, in ferment we first met,
eons ago, tiny microbes, evolving we were still,
yet love a flickering light, bound us together.
As two galaxies were merging fiercely then,
to turn us and our nameless pang,to worthless dust
there wasn't any time left for a future together.
In a microbial kiss pathos ruled,we melted as one,
promised to remember this tryst, imprinted so deep
wherever in cosmos we would meet in future
in whatever form we may be at that juncture.
                                        2.
This morning at the Metro that pang did revisit,
seeing you gazing at me in goosebumps I stood,
two galaxies within, I sensed were closing to to merge,
coming to my senses again I find  you've vanished
a microbe, you are in the air that every minute I breathe.

We will conquer time, go beyond, love has power infinite,
the encounters in flashes would intimate our impending union.
Dave Jan 2013
Floating
Formless
In warmth and tranquility
Silence
Eternal
Held here softly
I have no mouth
I have no eyes I
Have no voice
I have no purpose here
I'm just floating
Bumping into
All of the other
Meaningless life forms but
In having no meaning I find
Serenity
Calm
Peace

I think I had a dream
About something beyond our comprehension
Maybe something that held us before birth
Something just as blind as we are
Move in closer
I need to feel you
You seem so familiar
Where did you run off to?
What if I told you we're microscopic?
We're merely a conscience held within a vessel
Beyond this physical form we'll meet again
Swimming
Crashing
Through eternity
But right here
Right now
We're all just alone

Wait to reconnect with me
Let the rhythm bring you home
Tell me you will wait for me
Be patient
I'm coming home
st64 Dec 2013
the farewell of the magical-masque
           the dance of the whirlwind
           the twist in valediction
a pantomime of comedy dripping in life’s heat, its tragedy blooms forlorn
silently the mountain-ranges stare
the sky-face won’t relent and contemplates the open-disease in homes*


1.
disguised as simple relief – rescue lies cooing in the palm
     crumbling in blue-ash beside your grinding-palate
     you reach for pen and paper to appease an entity unknown
shrouded in grey, no scavenger can touch the head of one
who carries blessings in the scabbard – the present worthy of now

stairs are slippery, fish are mouthing, anger grows
     symbols hop along outrageous, so stylised and signs come in decisive
     all at once, almost
there is some purchase in the widening-valley
when climbing-feet need to rest on your narrow angular-will
and wait.. (before them chips rain down)
until the merry-turnstile comes in view


2.
the worm-wheel goes blank a while
and out tunes a dastard-and-devilish prank, courtesy of blunted-fate
sacred-fillies get hacked at by small silver things and they lie slaughtered on stark-plains
and the orb dips in reverse this time
a sooty-traveller from the western-flank
               glances out at massive-figures at supine-rest
               gets startled by the rude ***-fire
eyes slit and pates distort in hostile-fever
at the starling-ingénue in mock-fatigues and fake-epaulettes
but cheering up with wry-humour makes your feet
           a touch too slow to react in time
           and the halberd comes crashing down
well, the last thought you hold before your next one
is how utterly beautiful she looked at the station
long, black hair – silky-shining in your eyes and gay-dancing in the wind
when she passed you all her sweet-love from eyes so wet and smile so quiet
and selected dried-fruit in redolent-parcel
                                   a sealed pelt-skin of unmixed-whiskey
along with fresh-baked raisin-bread in cotton-cloth
                    coarse-sliced and buttered so generous
and
a semi-rusted dry-tin rattling its bounty of macaroons through that smudgy, ***** window
what sweet-victuals to keep alive . . .



man, that journey is a long one!


                             (I’M STANDING HERE        oh, you just know I am here

AND YES -- I’M WATCHING YOU                        
                                                                ­               and no use looking round now..
      YOU CANNOT SEE NOR HEAR ME  
                                                                ­               or begging a purty-release
                                                                 ­                                             
                                  oh easy, boy.. EASY!!)                                                          ­                            
                                                                ­                                             
                   ­                                          


3.
once more, the worm wriggles in microbial-distaste
and the season’s wheel comes dangerously close to being undone
IT DOES
and seconds later, cogs fly hard in every fool’s direction
and luckily.. you catch some in your face.. mouth agape
        crushing your tongue
        splintering all your dental-treasure
        smashing half your reason
no time for moaning.. or eroded-regret.. or even to feel your lips in ribbons
for, when they turn their backs, you will know
what to do..


because you’ve picked some pearls the hard-way..
that atonement could well appear in spells
of any shape
or size




not so?





S T, 30 dec 2013
beautiful in the mountains.. Jupiter enjoys the odd (but needed) breeze along with sweetness of Nature’s sounds  :)



sub-entry: ten times

you get ten times to refract your pain
mind your head now
the ceiling’s low
the parchment’s dry
and then some..

wait a little while.. it all comes round :)
Dalton Bauder May 2013
eye
in perfect motion, lost in time,
I subdivide the outer lines.
the enigmatic parallax;
dilation of the centered mind.

microbial in grander schemes,
my breath is born to exit me,
inhale the holy entity;
become the dreams
in vivid scheme.

intrinsic shapes of destiny
envelop my entire being;
a calculated entropy
that grants subconscious unity.
magnified, this smoke will bleed
into my every living deed,
tied into every breath i breathe
I stretch my being; exude peace.

I’m only what I dream to be,
as I ask myself to pray for me.
Bassam Mar 2010
Proceeding in the wake of mankind's scourge,
Spoken are the words of this great demiurge,
At dusk the cowled of the night shall emerge,
And convey a true evil on God's Earth to resurge.  

Unleashed and unfathomed, behold the words of a phantom
Turning cities into craters and the oceans to chasms,
Imagine: a picture perfect world, can it exist,
Without the plague of the human race, lost without a trace in abyss!

Ignorance tragic, the magic of bliss,
Static damage to the rabid on this planet of ****,
An example of this: the progression of time
Deteriorating in abundance, a final judgment for mankind.

Exterminate the population, man, woman and child,
Convictions, the arrival, apocalypse nigh!
None will survive, total disaster, blood stain alabaster
Abstain, refrain, salvation from a heavenly ******* shall be sought in vain.

Unexplainable cataclysm,
The missing piece of the puzzle unseen in catechism,
But it was written somehow and somewhere
And the emergence of its purpose was unclear, deny what you fear!

The end is near, malevolent seraphim invade,
The end is here, a feeble humanity kneels and prays
It was revealed, none prepared and none spared
And act of evil, fitting for the slaughter of a people.

Mephistophelian ascension,
A requiem for the souls of the ruined be sung
For a destruction, beyond all human comprehension.
Alarum with no human intervention!

An apoplectic annihilation, fed lies by inhalation,
Microbial immolation, infected detestation,
Evasive evasion, catastrophic, melancholic
Leaving mankind intoxicated by his own narcotic

Whilst hypnotically induced, the demons invade,
Equestrian quartet lead the massive evil brigade
A battalion of stallions, on fray to slay grace
Laid to waste in the face of the inhuman race.

To keep pace, without a trace, Messiah on Fire
In dire need, erase calumny the Heavenly liar feeds
Desire breeds and hatred grows
Within those a crueler fate chose the pyre to bleed.

An ascension to an unknown throne overthrown,
A crown adorned in thorns be thy Kingdom's scorn
To the Black, I am sworn, prophet to the swarm,
The scores of the forlorn born to battle in the storms

Of Ragnarok, the magma rocks rain from the sky,
The Earth will end in fire, watch the genesis die.
Terrestrial crucifixion, the mortals' last affliction,
Desperation bringing forth a dogmatic dereliction.

Infliction of pain, deadly diction to the slain in vain,
A spoken name, confliction causing friction
An addiction to the wicked, auspicious yet pernicious,
Foreboding a sinister outcome of ecumenical wishes.
**lyrics by Samuel H. Kelly for the Rare Form "All Will Suffer!!" EP, released in 2004.
Alexander Nelson Apr 2012
dont need relief from cluster headaches, hopefully i never will
i don't need pink blotting paper
i don't want anxiety to the point where I can't breathe
i don't want to rhyme anymore
i just want to understand why the man in the toll booth
annoys me to any extent
i hear something as i walk past him
maybe its his thoughts, or just the physical
presence, of him tapping the metal siding
maybe he's an introvert that's come out of hiding
maybe i just lied about not rhyming, i can't decide
i honestly can't decide anything anymore, it's beyond indecision
its bent derision of vision
it's beyond confusion, because the confused know that they are
im confused about whether im even confused in the first place
i am... urges, i am... impulse, im not...progress, or it seems that way
i could be progressing in relative terms, that's if einstein was right
but who the hell knows if he was
humans have been on earth for 5 million years, a drop
in the geological bucket, **** it
where's motivation when all collected knowledge
could "in itself" be progressing in the wrong direction
at that point we are the id and nothing more
we have nothing to offer microbial nature
on any other planets nomenclature, mars for instance
has a higher knowledge, their +1, we're -100
im just talking this system, god knows what's just 4 lys away
probably nothing, but nasa still wants to take more pictures of uranus
kiss it *****
smallblank May 2014
As you read this you are traveling 220 kilometers per second across the galaxy and I cannot stop thinking about the fact that ninety percent of the cells in your body carry their own microbial DNA and are not "you."

Which explains why your eyes likely originated from the belly of a star.

There is always a light at the end of every tunnel and if there isn't you should consider screaming until your voice echoes across the galaxies tucked within your irises.

I wonder if the trees know they must die every year for their leaves to become new again. Wounds line your heart like sticky notes left in the sun and the origin of you has been faded.

Black is the color of death but to your funeral I will wear white.

I will celebrate the death of everything trembling inside of you and stitch together funeral dresses for every version of you I watched leave without a goodbye.

I will wear white to your funeral to celebrate your rebirth soon to come.

Many hands will tie your old self to a chair and set the line between real and ideal on fire but only time can turn a flame into embers.

Most of the cells in your body are just empty space and skin is only a burial ground for old versions of yourself to die.

Your fingernails are only tiny shovels digging up a bed of dirt to plant new pieces of your DNA in.

I will cover my best dress in dirt and stain every white hem in celebration of the death of the fear inside of you and the birth of hope.
Being beloved must be pleasant
Depended upon yet carefree
What it must be like to deliver
Subtle nods, vague affirmations
Lacking regard of another’s interest
Seeing into you, from a skewed angle
Hoping to get recognition
While ostensibly unaware
Of the microbial ambivalence
Pervading the room from your eyes
Each morsel shared ‘twixt each
Whether intimate or scrupulous
Swept away with a shrug and a call
From a waiter and an ex
Like an invitation to despair
Robert McKinlay Aug 2013
I was wrong

The instrument I was playing
it wasn't out of tune

I thought it was something else

as I played offbeat notes
it revealed a beautiful monster

I was wrong

I thought it was something else

It glowed with spectral feelings
opening valves that should have been closed
so many facets to this being
I'm enhanced

I was wrong

I thought it was something else

I gave it air, I gave it my hands, I gave it my face...
I thought you were someone else
Who was I to be wrong?
Haven't I been playing you all along?

I was glad to meet you
I was sure you were someone else

Together, my beautiful monster,
I'll make you real,
glad you're here,
this was a perfect tune for us...
somber, majestic, synthetic,
microbial, pungent, diabolic,
colossal, all very near...
fixated on an elevated platform
we're lit like an attraction,
we're joined for all to see.

A beautiful monster,
I was wrong,
won't you accept?
Apologies.
I thought you were someone else.

We don't know how to play the song...
bold melancholy flows
tinged with mercy
sedated urgency;
I was wrong
departed we fall silent,
no longer recognize my face,
we both hear a tune lingering
no idea where it came from...

won't you accept?
you're someone else.
anastasiad Dec 2016
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Godrej appliances come in desirable designs as well as a wide variety. Trendy plus smooth on the lateral side, they are user friendly along with out there contained in the product. You will discover about three fundamental kinds of chillers available from Godrej: Border, Pentacool V2 and Eon. The three different types in turn, consist of strong awesome as well as ice free of charge fridges.

Exploding array amid Godrej refrigerators involves single home family fridges along with twin color surface finishes. Your Pentacool V2 sequence gives either single and double entry household fridges, hmo's displayed with shiny, vibrant summer colors. Ultimately, a Eon vary is often a high end variety including double home family fridges in eye-catching layouts and are available in numerous capabilities.

Godrej fridges are acknowledged to incorporate brand new and ground breaking technological innovation for all their versions. For instance your Great Shower technological innovation containing fridges with smaller channels made amongst shelves to help apply great air flow in the remotest crevices pertaining to complete taste. Also, this Anti - microbial technology, Norm Handle technological innovation as well as the ZOP know-how ensure that the chiller continues to be rust absolutely free along with inspiring seed no cost, hence offering a wholesome ecosystem in your case you.

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Robin MacCuish Oct 2018
I’ve stopped trying to please the people I cannot please
And in exchange I find myself looking to please me
I’ve stopped trying to find goals and achieve expensive pieces of paper
Cause I’m the end we are going to the same place proper.
Heaven or hell in dirt encrested ground
Embalmed or silent ash making microbial sounds
KM Ramsey Jul 2015
you know who i am
you have seen her
dancing in flickering candlelight
heady breath wafting the
sickly sweet smell of
too many consumed beers
drowning my inhibitions
inundating my irises and
letting my eyes betray my
carefully constructed façade
the grenade you throw yourself upon

but you haven't asked the right question

have you never wondered
what i am?

i am the tolling of
bells echoing through
deserted streets
cobbles screaming for
footfalls and bustling crowds
the only witnesses to the
belfry's solemn song
reverberating off the
business fronts boarded up
to ward off the reality of sobriety
and Death's march through the streets
sending the inhabitants running
disturbed dust blinding their
frenzied eyes
who search for a sacrificial lamb
as if a swathe of blood
across the door could
keep away such an
inevitability

i am the stars
but don't confuse that with
a confession
or profession of some sort
that i'm something infinite
for you to probe
with hyper-drives and
deep suspended animation
there is no alien microbial life
lurking below my frozen
absolute zero surface
i'm only the stars that
you lose track of as
you leave the blackness of
open space and enter
a deafening city where
skyscrapers obscure
and the pollution of
a million lovers' ecstasy
drowns out the light
wrought in the deepest parts of me
and catapulted through
the lightyears of black vacuum
only to be lost
choked out by incessant
revelry

i am the heaviness that
yolks itself around your shoulders
and the night black that
wraps itself around you in
its vicious velvet embrace to
***** out your breath and
envelop you
swallow you
pinch the flame
asphyxiate your existence

i am the tunnel under
the Pont de l'Alma
a loss of control and the
echoing reverb of
skidding rubber
tires whose
black smoke chokes out
the screams which constitute
the end and last breath of
a goddess among men who
never could understand her
and in her end
found culpability

i am the petrichor haze
that settles
nestles itself into every
corner of the barren graveyard
wherein lies my comfort
and my greatest hope
my fear of names and dates
and chiseled stone
and finality that means
a peaceful nothingness
that welcomes the most
effervescent
ebullient
peace
that comes with the
cessation of neural firings
and the end of all things.
letters to you i'll never send
brandon nagley May 2015
Lawmen oversee the old day's hanging's,
Exit signs designed only for those who wear worn out tennis shoes,
Conquered,
Overcrowding as if only cattle can fit through!!!!

No salt nor pepper to design creation meals of home,
Fall is near,
Plumbings far to clogged,
Days passeth night,
As night begins to freight!!!

Wolves on the outside trade fur's with ferrel dogs!!!

Clenching of teeth siren off as oven's they bake,
They brew,
Country town folk with rod and ungodly staff they overtaketh and rule!!!!

Crises of all temptation,
Bleeders to readers,
****** deviants get out to put down their own indignations!!!

Desire all thou wilt,
Desiree's,
Empathies,
Chalkers, scoffers , doctors of deaths pill!!!

Read on,
Read on uneducated pillar,
For thy hooks art thy scrolls,
Thy eyeglasses maketh thou gnomes of such readings to bring thou thrillers!!!!!!

Fragrant destiny resistant to all microbial force,
Accusation's humbling,

Sovereignty is a mystery to us mortals!!!!

Dragon's slayed to stature founder's ditches of war dug out of centurion portals,
Wreaking architecture drawn out of mapped whirlpools lies,
Some groweth deathly,
Sweet talkers are refusing to trust their own worried minds!!!!

Black coated tuxedoed hosts delighting their own escapes,
Some window's stay open,
Some stay closed in the fortress,
This inescapable place!!!!!!

Tis,
This human landfill,
Dump,
Waste!!!!
Anonymous Apr 2014
Lands all locked in cold and snow
Slows the news and consequences
But come the thaw
The floodgates loosen
And Spring has sprung
Minus all the illusions.

There's new life
Microbial and so talented
And the virii never slow
We're pretty under-handed.

But it's war that kills the most
Minus aid, direction or host
Ideas that were meant to be stamped out
Just keep making the post.

Killers die
Dreamers die
Flowers die
All awaiting
Another day.
Michael T Chase Apr 2021
...is like solving a crime that has left microbial evidence all over the city and in the adjoining ocean.
just saying
Ken Pepiton Sep 2021
heroes in our hearts don't look down from heaven,
I heard this old man correct an old cousin
prone to family history with hell escapees only,
looking down on us.

Catholic school through to BS, with a
mission in the spiritually minded flesh of a
20 year old in 1970, that was
sure time off purgatory,
if nothing more,

been there done that contests heat up…

don't you tell me where the kingdom of heaven is,
lest you contradict the king of kings theme,
pounding timpani's beat in me remembering 1972-
bah boom

from the future of then, this is no lie, it is an
after projection, after before thrown
in the mix, echo of a wow, actually redeemed,
for flavor, see real deal spice, functions best
in the presence of microbial ****
under your tongue, that convey the signal
through the channels,
to the gut that fires up the **** that clears the room.
Seriously, any middle schooler thinks it is art hilarity that farts wows.
Gutter Grimer Jan 2019
Plummeting underwater
microbial life fusing
humbling in complication
the crux of life
exhaustive in its display
ever-less grateful
as the hours creep
a desultory existence
awaits us all.
Satsih Verma Feb 2017
I had met the flower
after a longtime.
The rose.

And its fragrance
hauls me to childhood
after the big dying.

A tender, scented dream
will touch me,
to become a poet.

Lying on dewed grass
you think, a promiscuous
microbial libido begins.

The explosion will eject
free verses, waiting in silence―
to witness― the April fall.
Danny Wolf Mar 2022
Skywoman fell from her world above with seed in her hand. The muskrat, dead of life, clenched mud in its paw, its final offering so Earth could become. It all begins with soil and seed. Soil, a micro universe of life sustaining life. Seed, the tiny carriers of stories and sustenance. Two rich and sacred beings I will learn well in my life. My fingers have placed many seeds into cells packed with fertilized soil, placed many seeds straight into the Earth. I have watered them, transplanted their strong roots and promising sprouts, tended to them, harvested their food body and been nourished by their flesh. Soil and seed are the foundation of all plant life, and thus, the foundation of us. Their cells become our cells. Their fiber scrubs our bodies clean of waste and sin. They are the Earth's lungs that breathe life into our lungs. Skywoman fell with seed in her hand. Seed from another world, her offering to a place not originally her home. Turtle Island is not the home of my ancestors. I feel discomfort in the thought of tending to land that was brutally stolen. I find solace in the story of Skywoman. Through her steadfast dedication and reciprocity with the land, Turtle Island welcomed Skywoman in, let itself become her home by its own choice. Her offering of seed a promise to be its tender, its stewardess. Although this Land of Turtle Island is not the roots and soil of my Ancestors, we are all inhabitants of a greater Earth. Through the waters and the mycelial network buried under the old growth forest, I can reach to where my great, great, great, great grandparents stewarded land and tended to beast alike. Their stories are not lost to me, and although I may not know them in the form of words, they are, like the plants, the cells, blood and bone of my being. They comprise the very physical structure and spiritual essence of who I am. And so although this Land of Turtle Island will never be my ancestral home, I can only pray to become its native in time, by its choice, by its welcome. My ancestral home is Earth, as it is for all human life. All of the two legged beings that came before me have foot-printed her soft soil, swam in her rivers, and returned their naked bodies deep in the ground to be food for worms and microbes that digested both their skin and stories. These pieces of human life nourish the soils where wild ramps and fiddleheads grow, where wine berries burst in color, and where carrot seed roots itself sweet and deep. What are we but food for the impeccable microbial universe present in each and every handful of soil? If I work in this life to make my body, my flesh, my muscle, my blood, the most nutritious food for the micro beings to consume and put to new use when I am placed naked and free back into the ground, then I will have done part of my duty. May I one day be potent medicine for them. My duty, next to nourishing the microbes when my heart no longer beats, is to tend to this land as home, healer and relative. One day there will be land that I need, and it will find me, and I will work each day to know and tend and feel and understand that land like my own very body. Until that day, and still after, I will build upon my own heart and mind a beautiful layer of compost and woodchips to breakdown and become rich, soft soil. Soil that retains and builds nutrients and water, is beautifully aerated and loamy. I will build that world within myself so I can extend it outward to every seed I touch, every wild and cultivated food I harvest. And, when that land comes to allow me to tend to it, my offerings will be of humble, hard work. Of service. My work will be to become its native. May the birds know the beat of my footsteps like they know the beat of their own hearts. May the coyotes and the rabbits and the groundhogs and squirrels know my scent the way they know the scent of the wildflowers that have bloomed alongside them year after year, decade after decade. May the soil know the salt of my sweat that has dripped into its universe every day from April to October under the heat of the Sun. May my salts and electrolytes mix with their world, day in and day out, until they need me, too, to survive. May I be as integral to the system as every bee that pollinates the flowers, every frog that eats the bugs, and every fungus that consumes the dead leaf particles and turns them into fertile forest floor for the ferns and other fauna to emerge in ecstasy and vigor. The flavor of this place will be as diverse as the many worlds that collide and coalesce to create it. And I yearn for the day to know the shade of golden yellow of the butter that comes from the cream that separated from the milk that comes from the cow that’s been nourished by the land we have inhabited and fell in love with together. One day I will know just by the subtle change of the smell of the breeze that the magnolias and daffodils are about to blossom. I will know the sweetness of my carrots and green beans, the lingering smell of garlic scapes on my hands after plucking them in May. But first I must make a home of myself. First, my own body, mind, spirit, must be tended to with such adoration and respect and beauty and brilliance. So I will start there…becoming native to my own body. Becoming home to my own self.
Zywa Feb 2021
I take it easy:

fear and regret are like love –


microbial fuss.
Letters 760 and 764 (April 21st, 1889 till May 2nd; 1889, Vincent van Gogh)

Collection "Love Mind and Death"
his woebegone **** dental daze today May 5th, 2021

No particular rhyme nor reason
garden variety indentured flunky (me)
revisits his salmagundi salad days,
when oral blight smote
left front adult tooth,
which hellacious quandary commenced
when yours truly experienced
broken said central incisor.

Inxs of cold playing air
froze natural pond, where over head
Canadian geese (imitating
black counting crows) did blare
honking the latest goose sip loud and clear
when from behind a (Georgian) bush
(color of smashing pumpkins) did peek a deer

alert to any danger by parking
upright either one or both ear
lest predator doth lurk and induce fear,
while Harris Family and friends
oblivious among themselves
attired in wintry gear
which protection from cold
caused difficulty to hear

necessitating cupped gloved hands
to punctuate every muffled word
to be but barely heard
akin to talking with mouth full of custard
above the quiet riotous mirth
from this then gawky child nerd
precariously maintaining balance
on his skates heed glide like a bird
such attempts made
this boy didst appear quite absurd

ah, if only this mind of mine
did two step quick think
but woe misfortune awaited
across the bumpy natural rink
blithely jettisoning myself hither
and yon like a rolling stone going plink
unaware while in camouflage pose
disguised as one sneaky slippery fink
that snuck up in a blink

that found me squarely
face down shattering left front tooth
immediately discovered via
tongue as private sleuth
finding me in extreme agitated state forsooth
as if on fire from red hot chili peppers
wrought from jagged booth

winning sympathy from parents,
who did level best to tend distraught son
who ushered playback of events
with less disastrous rerun
praying for an angel
to grant reverse outcome brought none
gut wrenching grief
immediately terminated former fun
damage irreversible and
perfect white smile forever broke.

So much of my precious existence since
found me rooted with mouth ajar
as sigh asper the dentin-cementum
so mud dear reader (with dem perfect
enameled pearly whites), aye har bar
envy for those with a complete set

of eight incisors, four cuspids (i.e. canines),
eight bicuspids, and twelve molars
(including four wisdom teeth) tabulating
many hours in the car (engendering
saddle sore bony tuckus)
plus regarding chunk whereat,

pernicious cementum funk
viz distraught psyche,
when muss self as a lil monk
key decades after being examined
by family dentist Doctor Marcus (NOT WELBY),
excellent practitioner (button irate pulp pill

people, especially children) hater –
the grinchy, grouchy, and grumpy,
whose private practice located
in Levittown, Pennsylvania,
and when prepubescent self underwent

pertinent more explicit focused
intense noninvasive procedures
asper subsequent cause of speech impediment
determined why air didst jump

thru nostrils, (speech therapist
at Henry Kline Boyer),
neither thin nor plump informed parents
of Lancaster Cleft Palate Clinic –
fifty plus miles one direction),

where chief prosthodontist (the curt
Doctor Mohammad N. Mazaheri, DDS, an Iranian
whose expert reputation,
sans strict manner didst trump
his aura, karma evincing clipped commands
forceful as a vocal whump

before launching into meat and potatoes
of crux comprising real aim
constituting modus operandi
(and cresting away from details indirectly tide
into main intent, nobody aye blame)
for thine dental debacle quandary

(managed by gumpshun,
whereby eons hyperbolically
toted beyond google),
and despite optimistic stance
wool worth anesthetized numb skull claim
nascent malocclusion faintly affecting,

hinting, pointing toward Periodontitis
(despite diligence attending
to oral hygiene frame)
the manifestation
of major looming crisis compromising,
forgoing, instigating, et cetera loss of teeth,

this (after agony in league with separate occasions
twice wearing braces, concomitant extractions
of wisdom and removal of crowdsourcing –
close up toward the front of mouth teeth - game
some microbial bacterial
agent provocateurs didst maim

self-acceptance, and (found thyself
as a boyish twenty something
weathering onset of gum recession,
maxillofacial surgery, impressions,
x rays galore, scaling)

necessitated (score years later) urgent intervention
i.e. treatment plan under auspices
re storied name
University of Pennsylvania
Dental School to mitigate malady

entailed every last tooth plucked with ease
since no other recourse could tame
accompanying jaw bone loss,
which destabilized rootless choppers,
and despite the state of the mind turning to pulp
(this haint no “fiction, nor FAKE)

thus I acknowledge sincere gratitude
vis a vis thru poetic aire
for the entire fleet of dental students,
and staff that didst care,
who assuaged distress,
exceeding the best expertise flair
which eventually warranted

being fitted for dentures here
bringing an exemplary end result
encompassing yours truly writing in his lair
after about a dozen years encompassing
so many wing (bitten) angels far and near
across webbed wide world to help repair

chronic distress minimized now, cuz there
prevailed the most blessed delight
when Medicare picked up the tab
now smile more willingly
with artificial dental wear
donning blitz end until
mine last mortal year.
winter Apr 2022
oh to live a life with journey and
not these
microbial sparks
little waves that seem to spark through
like a cobweb soul
painting geometry i can hardly
understand
abuzz with Autumnal thrum

Divine myriad biota amidst
heavenly Lily of the valley
(Convallaria majalis),
he didst imaginatively greet
Edenic heavenly terra
incognita immeasurably sweet

nature's ensemble proffering
Gaia's quintessential orchestration
resplendent sensational treat
natural splendour regaling,
this fellow wayfarer
happenstance gifted autochthonous peoples

espied proud specimens unobtrusive
planted armada, viz sleek bodies fleet,
of foot while me accidentally
risking, schlepping, traipsing... offbeat
winessed unschooled tribe,
yet verily synchronized,

primed, muscled... athlete
their soundless rhythmic swiftly tailored
flit to and fro upon poetic
unshod calloused feet
carefully, gingerly, lightly...
I shod dully tread nsync

toward drumlins upbeat
mouthing, kneading, imbibing... glorious
ebullient choral unadulterated feat
extemporaneously kickstarting crisp and neat
pow hour full rhythm across
analogous macroscopic excellent spreadsheet

inducing their sonorous symphonic
roundelay unfamiliar tweet,
whereby flora and fauna future meal to eat
oblivious regarding mine seat
dated existence, which quiescent aesthete,
yours truly basked,

froliced, luxuriated... complete
as once innocent hymnals kindled atrocity
this observer, spectator aghast white as sheet,
how civilization's machinations didst deplete
terrestrial firmament within one fell stroke
eradicated once pristine unbroken

promises chiseled to cheat
rightful owners expansive swath
over yonder til ocean and land did meet
Europeans scoured seas one after another
lumbering bulwarked fleet
exhausting resources while simultaneous

mowing down aborigines
grotesquely analogous harvesting wheat
indiscriminate deliberate genocide
decimating indigenous tribes beat
defenseless against microbial
weapons of mass destruction,

thus only within third blind eye
courtesy invisible paleface with tenderfeet
strictly envisioned Perkiomen Valley
once abundantly populated
with ample game during cold and/or heat
paradise unbroken stretched hinterland,

where place names mock to pay hollow tribute,
where native peoples no longer replete
vinyl city amidst amidst graveyard
lovely bones turned to dust
paved over by mainstreet.
without yours truly experiencing urge to defecate

Neither bloating nor constipation
arose, but one nondescript anguished
logophile anticipated intestinal blockage,
hence prophesying worse case scenario

I (predicated in direction for an adult)
ingested three Dulcolax tablets
and subsequently this joker
found himself riddled
with debilitating diarrhea dilemma
that afflicted me hours on end
today April 11th, 2022.

Severe **** travails
immediately brought forth
****** recollections regarding
my offal bout with shigella
circa approximately July fourth holiday
nineteen hundred and eighty seven
which ranked as worst pain in the ****.

Two women (Ziona and Susan –
surnames unknown),
plus your truly, which trio
befriended each other
from Thursday night contra dance.

Aforementioned women
minimally identified
via first name basis
(since their sir nom de plume
unknown at this juncture),
and yours truly linkedin,
via above said social venue.

This piece of information mentioned
since that innocuous site
(Summit Presbyterian Church
at Green and Westview Streets
within Mount Airy, Pennsylvania)
essentially served as
figurative starting point
per following true anecdote.

Though about thirty five years ago,
my memory easily jogged,
asper unforeseen brush with death.

Innocence and naïveté twin factors,
tangentially contributed to agonizing ordeal.

Ask me what I ate for breakfast
this morning, and ye will receive
a blank stare!

Turn back the hands of time,
well nigh eighty percent
since existence sans mein kampf.

Every summer informal conclave
regarding congregation of hippies
(most of whom found me
looking conservative – fully attired)
decide upon a locale communicated
thru the grapevine.

The Rainbow Family Gathering
(vast majority opting to shuck clothes)
that summer held in Cheoah National Forest
just outside Robbinsville
western North Carolina,
and the farthest south
clandestine group congregated.

Among the estimated 12, 700 attendees
(one of whom included yours truly),
more than fifty percent
of the sprawling masses
contracted the near fatal
outbreak of shigellosis.

Sanitation in the expansive campsite
ranked as poor to non-existent.

This absence of proper hygiene
provided opportunities
thru contaminated food,
water and person
to person contact.

Widespread transmission
of the very opportunistic disease
caused by Shigella sonnei,
resistant to ampicillin,
tetracycline, and trimethoprim
sulfamethoxazole;
the rapacious organism
categorized as colicin type 9,
and contained a 90-kilobase plasmid,
not found in non-contagious related strains.

Misery loves company,
and this epidemic plagued
the infected with unrelenting diarrhea
and general lower
gastrointestinal debilitation.

Unsure how this then
late twenty something singular male
managed to weather
the microbial onslaught,
which rendered me prone
to a doubled over spate
of being lobbed and hamstrung
hammering the lower gastrointestinal tract,
and incessant severe loss of nutriment
vis a vis thru ****** a steady state
quintessentially unable to nourish
an already slim body.

Upon relieving chronic purging
from the sphincter muscle,
the sensation to expel
countless times thereafter
explained (not recommended diet)
the rapid loss of weight.

Whence returning home
at that time 724 Level Road,
Collegeville, Pennsylvania, 19426,
this ghostly appearance of mine
immediately alarmed, discerned,
and gauged family members
to schedule me for
immediate medical intervention.

After being examined,
(what little flesh remained
of what resembled
a Holocaust Concentration camp victim),
the physician could offer
no pharmacological remedy.

As the days of my life passed
under bedside and medical watch
of mother, who worked
as a licensed practical nurse,
yet could do little or nothing
to halt her own demise courtesy
metastatic carcinoma viz
ovarian/uterine cancer
approximately seventeen years ago
early May 2020.

— The End —