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Àŧùl Dec 2016
Preventing contamination,
A constant challenge in cell culture.
Contamination not only affects,
The culture in question and,
Costs time and money,
But also endangers the reproducibility of results.

No cell culture problem,
Is as universal as that of culture loss
Due to contamination.

Generally, contamination may be separated,
Into categories of microbial,
And eukaryotic contamination.

Examples of microbial contamination include:
Bacteria (including Mycoplasma),
Fungi and yeast;
Eukaryotic contamination includes:
Cross-contamination with other cell lines.

Bacteria, yeast and fungi,
The three more common types of contamination,
But luckily these forms are often detectable,
Under the microscope and,
By visual cues,
Like colour or turbidity changes in the medium.

Mycoplasma is a small genus of bacteria,
That lack a cell wall and for this reason,
They remain unaffected by common antibiotics.
They are also difficult to detect,
With standard microscopes,
Due to their size, about 0.1 μm in diameter,
And the fact that they often attach to host cells.


To prevent contamination,
Use 70% ethanol for disinfecting,
Equipment & surfaces,
Related to cell culture.
Sterile filter the media first,
Before bringing to the lab.

Fetal Bovine Serum,
A potential source of contamination,
Contains mycoplasma.
Filter it at 0.1 μm, or,
Gamma irradiate it.
Aseptic technique,
Necessary.

The laboratory workers be the last,
But not the least source of contamination.
Teach them the ideal laboratory practices,
To ensure asepticity in a laboratory.
Source: American Laboratory

For revising an important topic from Animal Cell Culture.

HP Poem #1299
©Atul Kaushal
ryn  Mar 2016
Forgiveness
ryn Mar 2016
Grant me forgiveness.
For my mouth had acted prematurely
and erred.
Acrid words my tongue can't retract.
My lips quiver,
pursed and scared.

Grant me relief.
For my ego had lunged.
Fueled emotions that strayed.
Sensible thoughts in mind
that my heart had betrayed.

Grant me strength and courage.
Let the next morn's sun,
illuminate the dark obstinacy of my heart.
Allow this bitter turbidity to pass.
So I could walk the hard road,
to a brand new start.
.
Sometimes words carry more venom than fangs.
And often, the path to absolution lies first, in forgiving oneself.
.
ryn May 2016
.

"Quieten down...
Release your anchor, and sink into bed."


"I can't...
The whims of the world are much to heavy...
For me not to bother."


"The weight of the world isn't yours to bear...
It'll sort itself out,
if only you'd give it time to spare..."


"But that's just it, isn't it?
If only there's enough time for all of it to fit.
The ******* truth is...
there's never enough.
There can never be for those built with edges so rough."


"Why are you so sure
about something that has yet to happen?
When future's sand has yet to be spilled,
and its ink has yet to be written."


"Because that's just me.
I am a being fraught with worry.
You know that.
It's the only way I can be ready.
It's the only way I can be steady."


"Then allow me to keep you company.
For I am you, as much as you are me.
Till such time you eventually feel,
that you're ready to retire and heal."


"Thank you...
Your words comfort me much.
I welcome you,
to see me through this chaos in my head.
I've severed the anchor...
Let us sail to tranquillity,
leave the turbidity in our wake.
And replace it with
peaceful dreams in its stead."




ryn
ryn

.
You know you've lost your marbles when you write pieces such as this.
.
Tessitura, psalms, and songs of praise, they branded atheism when singing Christian psalms in the streets making ineffable groans, where the exordios looked from the back with Delphic prose, where the dart that opens the curtains of the hallelujah tormented, with darts that rubbed weathered in the tentative to rise of the stores of Sanequerib. They are relatives of Incipit Psalm 69. " Saint John said as they continued to climb the Calvary of Profitis Ilias, but this time in the company of the Help of Isaiah, with a great spirit of being from the cavern of Elías in Haifa, at a flat point at the time of the Benedictus. Already the Assyrians were returning the same way they came, as Isaiah prophesied, in the morning with ejaculations that ended with the crass rottenness that could end the day without a step other than an anti-Jesuit one. Prayers go and implore the Omnia Vanitatis, the moment when the sun honors, taking you towards the close of the day with the perpetual antiphon. The vigil was reaching the lines of Isaiah does not rest, in Trinitarian doxology. Where is the darkness, where is the glory to see you...? If the stars collide with each other in Baptismal frowning, and in the mystery of Vernarth that lies a complex, tied to becoming that never begins, and what was Christic history of a morning introit.

Saint John the Apostle and Vernarth express in the Trinitarian doxology: “Through Christ, with him and in him, to you Almighty God the Father, in the unity of the Holy Spirit, all honor and all glory, forever and ever. Amen"

The triangular taxias of the Hetairoi made faunas that came cutting themselves with the wind of the "incipit" of Psalm 69: "My God, come to my aid;" Lord, hurry to help me ", by the Keras or wings of the site of Arbella; or Gaugamela rather said…, sonnetized by some Pazhetairoi, made up of 32 Syntagmas, as units of sixteen revived Falangists from Court V of the Helleniká Necropolis, bilocated on Patmos, a few feet from the Mandragoron project. Thus the triangular spellings of war were formed again, to the astonishment of all those present. Alexander the Great, already graceful, was over-trained in irrigation and supplications, he was consisting of 128 Syntagmas, with 62 Falangists covered by the Cinnabar that subdivided them into bones by sixteen of the Lochoi or guides. The Syntagma bipartite was enlarged by two Syntagamatarchos captaining two units, all with their semi-open belly, re-liquidating their viscera by the Ghosts of Shiraz, the Saltimbanqui Hydro comes from Roknabad (also known as Aub-e Rokní), from an underground channel which carried spring water to the city from a mountain located ten kilometers northeast of Shiraz. Here he has to mend the propellers and water ropes to do his acrobatics on the water, with greater songs in the poems of the Poet Hafiz. When he bites his tongue, they repair it with the verses of Hafiz's Koran, there are three hundred creeds, three hundred hectares to irrigate with his wheel the sadness of those who cannot have the gift of the rivalry of Montenegro and Monte Blanco, to overestimate the liveliness of the caravan that trembles with uncertain doubts here on Patmos "

Saltimbanqui of Bascule says: “We are Epi ghosts, green in reverie with tutelary ropes, to jump through the trapeze of the photometric units of the heavy Almeria of the highest Mirror of the Sea. Will take you back to Limassol. Curiously to the same ship as the Eurydice that sleeps in the swings of the sea, and in the arms of the petulance of Dionysus in a new awakening of lethargy of theorization of the superstrings of Anaximander, here is the intrinsic speculation of science, already that this is not just purely empirical research. "

In between them, they form even and odd rows. The horizontals were tinged with the Red Blood cells that became volatile and surrounded the Xyston lances, for thirty soldiers of the Diloquia, with their dismembered arms that began to take them back with their hands tightly girded by the song of the Theological Shemesh of San Juan, which subsequently rescinded last in the sum of two taxiarchies, constituting a Syntagma. The units rose with the sickle that cuts definitive death, to reconstitute it in five thousand that should tread through the hierarchies of formations, amid the frolics of the Phalanx, where Vernarth protested to all “Khaire, Kalos irthate apo tin kentriki, Welcome from Hell !"

Thus the Phalanx was constituted among the Syntagmas in metaphors of the Falangists. In this way this antiphon was revealed martial, denoting synergies of the Sybilla Herofila that conferred to the world of Trinitarian Doxology, among ashes that remained by a solid cobblestone witness of the reluctant troops that testified to the sense of interpreting the law of bringing to the world what to their lives it owes them. The prophecy shone from an intangible Isaiah before all in this concomitant episode, and to the degree of the reign of Judah, here together with the prophet Elijah, they faced the hardened fragrances of blessing as oracular teachers of so many goods, and of the benefactor that protects by inspirational mandate, making laws for the end times before closing his own eyes without having prophesied them.

The rows in “V" contrasted with the corridor friezes in the crowned troops of the Hetairoi, and in the syntagmas that became appressed from the triangle that opened the three-quarter proportions of Athenea's physiognomy in Pergamum, subjugating Alcineo, so that finally it was forged in constellations of equanimity in the fifth courtyard or "V" of the Necropolis of Helleniká in the allegory of Vernarth, stopping the plausible dogma of the initial that glosses the Law in Vernarth's "V". This in turn in double syntagm of the Syntagamatarchos guide, in the high sky of Patmos, and in the medrones growing on the antlers of the proclamation of Wonthelimar, which made them a twin "W" in the star that shines in the medrones of the Ibix, in the Cornacabra and in the Cornucopia, with certain docile movement, adhering to acrostic and prehensile preliminaries of the Isaiah saying.

The Phalanx Alexandrina Heterochromatic of Alexander the Great volatilized between the villi of his Falangists, climbs the Holm of Zeus and causes a "Gore" or horrifying reflection, allowing the rhizomes to become a hundredfold, which will make the nominal order of five thousand, for each member of the Syntagma, in an astonishing quantum that reproduced itself to materialize before Him. Then he tied each one of them as Prometheus chained to each of the oaks, from an Akane grocer, incontinenti withdraws a sharp dagger and opens each one's veins to free them from the isolation of so many years settled in their last heterochromia of the War Iridium that he conferred on them, to endure the visit of the spirited Grim Reaper. This causes liberation, in this way they re-install themselves in their bodies, with Iridium or iris that made them see before their optics in two biases of Hoplite alter egos, impacting half of their body. Alexander the Great, being the philanthropic heir and of Platonic legacy, made them superfluous in the melanin that fell from the Epíchisis or libation vessel, to taste the effluvia of Dionysus with the maenads, with wide ambivalence filling them with viticulture, so that they would flow through the veins of his soldiers, and to revive them with the Dionysian must of melanin to the left eye of the Hegemon King Alexander the Great, with Jasper in the left, and the right with ultramarine from the bottom of the Ionian, on the banks of the washed banks of Patmos, in high swells of Greek alcohol that was distilled from the Mosacism of the stones when unraveling the peripheral forces from the prefectures of the great native of Pelas. They ordered areas of all Greece under their heterochromia flow that gave life to the Perifereoaki, or periphery for Central and Western Macedonia that came with great vigor, with Epirius central, western Greece, Peloponnese, and Crete. East Macedonia and Thrace, Ionian Islands, North Aegean, and Thessaly, later they would go for the Aldehyde alcohol that summarized and epitomized Dionysus taking him with four eagles that distilled the unprisoned Syntagmas of the lines of 16, 32, 64, etc...., for purposes never to start on an omega all the way to the Ionian Islands from Corfu.

Alexander the Great, went near the pre-urbanization of the Mandragoron towards Vernarth, somewhat dizzy, and before attending to him he presented himself first to the Zefian; who looked at his iris like a foreman who re-divided his visuals, by prevailing in eagerness to restore his soldiers, to help in the construction of adventures of life, and to assist in building the Megaron, which still rested in the myopia of mythological vision of the Gods tied in animosity with the Titans. Overwhelmingly, he highlighted the clouding or turbidity that was seen beyond the radius or visual field of two realities, found in visual refraction and interference with refractive statisms of the periphery that led him to the other world in Babylon when death imprisoned him...? Here the root revived, it became parallel in a unique world with divergent lights, which entered his Akera or right-wing of his soldiers, bringing visual acuity that brought the perchlorate volatilizations that hovered in the boots of his soldiers, when they marched in awareness of the retina and of the mean light, that for the first time was clarified in true holistic and political from a Parthenon with the musk of mortals and immortals of neo Hegemonic ophthalmology, which he was already re-leading by his command, where he was going to invest his greatest and most spiritual elemental Commander Vernarth, with his Himation.

The rays of his eyes seemed distant, but they were diffuse and alternate, they wandered through the lens of his clouding, which blinds a partial of the left Akera, or flank of the Hypaspists that dazzled Parmenion. Here the optics of Alexander the Great, remained in the diatribe of the small eye next to another that was enlarged, being hyperopic of a mysterious confine in the severity of Dionisio when confronted with him, in light effects of the high liquid vineyard, refracting meridians in his troops next to the Hexagonal Primogeniture who observed them behind the magenta image, which was the one that flashed from the Clouded holm oak and eclipsed by calm heat movements, and rising air masses that were in the opportune station of good sense. When being aided by the Maenads and the Herophile, they were teaching from a parent, who now sponsored the entire political and spiritual will of the Hoplite side, made up of the King of the World Vernarth, together with Alexander the Great, after receiving the photocoagulated lightning bolts. of the officers, under redeeming and reduced of the metabolic, and of the oxygenated preeminences of new lungs for each devout consecrated body, towards Saint John, the Apostle, pigmented and mechanized with aggravating heterochromia, and extensive in the bodies raised in new parallels that have to confront an anonymous or semi-god by turning for his own.
Antiphon Benedictus III Isaiah / Syntagma
All birds of my heart were high in sudden curved of the azure sky where Enthusiasm and happiness flourishing directly in the magnificent immigration through
dance of wind and wine.  
Swirling
and
Swirling
From north to south.  
showing amazing absolutely, by exposing colorful long tails
from earth to clouds where human’s eyes rarely can magnify
How far? …
Don’t remember exactly
It was fire, water or turbidity dust of shapeless tornado in
unexpected curved of the grey road
that
All birds of my heart …all birds of one type
suddenly lost.
I...
Reluctant, morbid…feeling false
I…
in hours extend in duration of long time burn the  expectations
as outcome,
waiting for
magnificent immigration may return them to their nest, on top of my heart.    
maybe they die, maybe go far…
nurturing and nourishing them a lot,
how is it possible to stay without birds of height?
while
“Maybes” …has never grown any flower in any dust.
while
   while...
the next immigration is not so far
… birdies…
Shall I see you again in the realm of my emotional yard?
Ghazale#Ebrahimzade#
Muharram 2017 #(Ashoura Night)#
Dirt Witch  Nov 2018
Turbidity
Dirt Witch Nov 2018
Swelling water
Pours heaviness of limbs
To the swallow of sleep.
Rain-water fingertips
Dampen the sheets,
Moisten cigarette papers.
A tobacco spire
Breaks the clouds of my teeth,
Announcing holy fog
In respiration.
The sun drowns at 4pm
In a pool of deadened leaves
Choking bubbles of light
Through eternal perspiration of winter.
Liquid abyss soothes the sky.
I rain until morning.
Sourodeep Jun 2015
You thrive in my heart and mind
as waves of life, go up and down
no amount of gold in a chest
needed to strengthen our bond by any kind

As kids, each day had eventful moments
things we had fun with
streaks of silly happiness, added to life
all flavors of spices and condiments

Pulling each other's legs, on stupidity
fighting, and racing on our cycles
betting on idiotic facts and ideas
but supporting each other , in life's turbidity

We went our ways ahead
molded ourselves in different worlds
though separated by miles, we were just a call away
hearing your voice, a simple reason to smile

In those times, when things look so bleak
clouds of trouble and confusion covers us
not knowing where to strike, which door to knock
you were there for me, not letting me feel weak

The joy of success
the urge to share, was
always with  you
far, yet so near

They say with time,
people change, but I know
you will value me
our friendship, much more than any dime

When this journey will end
at the beach, watching the sun set
silently, melting these life's memories
I will be glad, that I had you all along
as my precious **friend
Dedicated to all my close friends, wherever they are now.
Damaré M Aug 2013
If you check the forecast
I bet you wouldn't figure that all of the people who were once starring in your life will later voluntarily accept a cameo role

They'll cast the brotherhood right into the wind
No matter the degree of the cordiality

If you check the weather now the radar will predict clear skies
Eventually there will be turbidity and heavy rainfall
And a thunder storm that will send all of the forged people striking

You'll be cold as hail
But you'll rather be cold as hell
Before you be hot as pandemonium surrounded by counterfeit persons

So I encourage you to check your forecast before you cast your life with beings who's used to being trife or slight

If they're unwilling to walk with you through your tight fog
I will give you an insight
Don't allow your eyes to precipitate
Monitor your satellite
And it's so SAD about life
So you'll have to condense your map
And don't allow everyone to make the legend
Phi Kenzie  Oct 2018
Hurly Burly
Phi Kenzie Oct 2018
Observable words
turning in circles
perfectly working
affirmed in impermanence

Serpents within swirls
swerve in the verve
curvature burned irksome
turbidity skinned earnest

Journal pearls quirked
turpentine turbulence
since worries serve nervousness
the cure in spurts of churlishness
I live vividly without visibly having the ability to live willingly nor the versatility to fight your volatility. Unequivocally I believe in relativity but unofficially I use negativity as a means of self-sufficiency. Naturally I have a proclivity towards acting predictably when publicly judging turbidity. Additionally I hide in anonymity and indignantly ignore my epiphany of the asymmetry of unanimity. Shamefacedly I turn to your intricate dystrophy and observe the futility of my soliloquy. I can' find nobility in dying deliberately, but it shows efficiency in skimming humanity. Initially my hostility was untangible but it has suspiciously aquired solidity and is now intermittently sending signs of my eccentricity. My alkalinity is running low because surreptitiously the pungency has grown. I am undoubtedly peripheral to the society and irresistibly disposable in the industry of this idiosyncrasy.
Mayday , afternoon turbidity , aeolian dynamic flurry with cursory airborne splinter , tall tunic Pines release their last remnants of Winter , at the cusp of torrid June with wind-borne , whirling , stern delivery
Copyright May 14 , 2016 by Randolph L Wilson * All Rights Reserved

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