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Àŧùl Dec 2016
Preventing contamination,
A constant challenge in cell culture.
Contamination not only affects,
The culture in question and,
Costs time and money,
But also endangers the reproducibility of results.

No cell culture problem,
Is as universal as that of culture loss
Due to contamination.

Generally, contamination may be separated,
Into categories of microbial,
And eukaryotic contamination.

Examples of microbial contamination include:
Bacteria (including Mycoplasma),
Fungi and yeast;
Eukaryotic contamination includes:
Cross-contamination with other cell lines.

Bacteria, yeast and fungi,
The three more common types of contamination,
But luckily these forms are often detectable,
Under the microscope and,
By visual cues,
Like colour or turbidity changes in the medium.

Mycoplasma is a small genus of bacteria,
That lack a cell wall and for this reason,
They remain unaffected by common antibiotics.
They are also difficult to detect,
With standard microscopes,
Due to their size, about 0.1 μm in diameter,
And the fact that they often attach to host cells.


To prevent contamination,
Use 70% ethanol for disinfecting,
Equipment & surfaces,
Related to cell culture.
Sterile filter the media first,
Before bringing to the lab.

Fetal Bovine Serum,
A potential source of contamination,
Contains mycoplasma.
Filter it at 0.1 μm, or,
Gamma irradiate it.
Aseptic technique,
Necessary.

The laboratory workers be the last,
But not the least source of contamination.
Teach them the ideal laboratory practices,
To ensure asepticity in a laboratory.
Source: American Laboratory

For revising an important topic from Animal Cell Culture.

HP Poem #1299
©Atul Kaushal
King Panda Jul 2017
a haw and saw.
a thorn.

fruit: it is ecstasy
never bit and
undeniable.

you slurp—a cat licking
its paws
ruby and clear.
moth and cloud
drape over fruit,
make up sparkling nectar.

love is sickening.
you spend five dollars on
a rose at a bar for
a girl you will never see
again.

she will take the flower and
throw it in the trash
outside with the hundreds of
other roses.

no matter.
they have fruit, and fruit
concludes. it is life
cut with claws.
their beauty, seemingly to
be always in the clusters above.

*******, rose. **** your dew.

they seem to say. that’s when
the light hits and microbial
bleeds to miss ruby.

JAZZ!

at night retrains
beauty, makes it edible.
the rose, changing the color
of its dew—black pearl in
this drape of mystery-shaped
night.
Anonymous May 2014
"And now please welcome today's anti-terrorism speaker, Anonymous!"

[anonymous applause, dwindling out]

"Thanks, everyone. The reason I prefer anonymity should be self-evident, but just to make it clear, I wish to avoid the recrimination of the hostile element."

"Before I got here I was just reading, and believe me I'm still not believing, but it would seem, on the whole, that planetary aggression is on the slow."

A hand is raised
A hand is ignored
The speaker moistens his lips
Prepared to emit a bit more.

"I have stats and stories
Tortuous anecdotes about little girls and boys
Food and sanitation is a crime itself
And I'm prepared to say we live in our own hell."

Arms upheld wither down
As new hands reach for attention
But the speaker ignores them all
Intent on his own presentation.

"The reason for hate
Is more or less clear
We fiercely believe one thing
As they devoutly believe another.

But do not fear!
We are right and they are wrong
They saddle their own children with a death song
No cartoons of basic morality
Just legs with bombs
Made to go off remotely."

An angry rustle
Amidst lowered hands
Quieting now
Like they're getting the hang of it.

"Humans are robots
Programmable, malleable and sometimes trustworthy
Highly complicated machinery!
Indoctrination is the virus
That seeks to destroy the outside."

Again the raised hands
And eyebrows too
All these fluttering robots
Fluttering in a pseudo-free zoo.

Ignoring the obvious
The speaker plods onwards
But modulates his voice
Against their trained reactions.

"We need to accept and enfold
An ideology only thousands of years old
To mutate and twist
Into what our children might wish."

Someone yells "Disney!"
Another mutters "Black whiteys"
But there are a few
Who remain to hear it through.

"Despite what you think
Despite who you are
Against all you've been taught
We've come quite far.

You may not know your son
You may not know your daughter
But leave them alone
And tomorrow may happen.

Put the guns aside
Drink from your hidden bottles without shame
You are who you are
And you should let them be them."

This is not what anyone wanted
Anyone over the age of ten
This is not what anyone wanted
With children and the urge to brainwash them.

The room trickles out
Leaving the most devout
Devoted to the future
Any future left standing.

But amidst this group
Are hard-liner elements
And one has a voice
Cutting through it all
To ask, "What about bomber babies?"

And riding right on top
Is a fat slobbery Republican fop
Demanding in his self-entitled way
"What the **** about America?"

The speaker shrugs
As if to indicate
Which question
Is more stupid.

"We seek to leave the planet
And develop tech to make it happen
You go your way
And we go ours."

The room is smaller now
They indulge in eye contact
Personal communications
Words, hands, heads and eyebrows.

The speaker sighs
As if on the cusp of absolute honesty
Then spills his true guts
To these few radicals and emissaries:

"Our worst enemy is ourselves
Through millennia fashioning our own hells
Subjugation of non-prominent DNA
Believing destruction will pave the way.

But on a not-much larger scale
We're just cheap entertainment
For every other race
That crawled up this hill."

The crowd is slightly subdued
Probably more from shame
Than anything
Because shame is in the DNA
And experienced by everyone.

But we can always rely
On some fat Republican to decry
"But not me!
And for sure not my children!"

And now even more file out
Hearts emptied and minds afloat
Now it's just the sweating speaker
And a few odd haters.

"We're a microbial phenomenon
Miraculously still alive
And still inept
At staying alive."

He waves a casual hand like a maestro
And behind him the stage glows
A 30x30 screen descends
Illuminating bugs as they crawl.

"We're slightly smarter
But no more hardier
Than Hymenoptera
Except we can leave this planet."

Red-faced and obviously insulted
The old fat plushy storms out
Leaving now just a few
To adopt this future-flung view.

"We need to terraform and colonize
Sure, and design space suits
Pleasing to the eye
But ultimately,
We need to get the hell gone."

One clap, one frown
The speaker shrugs
As if wondering
Why aren't we all gone?

And so he is left
With the clean-up crew
And one fruitcake
Who asks
"Will God come with us?"
K Balachandran Jan 2016
1.
On a nameless planet, in ferment we first met,
eons ago, tiny microbes, evolving we were still,
yet love a flickering light, bound us together.
As two galaxies were merging fiercely then,
to turn us and our nameless pang,to worthless dust
there wasn't any time left for a future together.
In a microbial kiss pathos ruled,we melted as one,
promised to remember this tryst, imprinted so deep
wherever in cosmos we would meet in future
in whatever form we may be at that juncture.
                                        2.
This morning at the Metro that pang did revisit,
seeing you gazing at me in goosebumps I stood,
two galaxies within, I sensed were closing to to merge,
coming to my senses again I find  you've vanished
a microbe, you are in the air that every minute I breathe.

We will conquer time, go beyond, love has power infinite,
the encounters in flashes would intimate our impending union.
Dave Jan 2013
Floating
Formless
In warmth and tranquility
Silence
Eternal
Held here softly
I have no mouth
I have no eyes I
Have no voice
I have no purpose here
I'm just floating
Bumping into
All of the other
Meaningless life forms but
In having no meaning I find
Serenity
Calm
Peace

I think I had a dream
About something beyond our comprehension
Maybe something that held us before birth
Something just as blind as we are
Move in closer
I need to feel you
You seem so familiar
Where did you run off to?
What if I told you we're microscopic?
We're merely a conscience held within a vessel
Beyond this physical form we'll meet again
Swimming
Crashing
Through eternity
But right here
Right now
We're all just alone

Wait to reconnect with me
Let the rhythm bring you home
Tell me you will wait for me
Be patient
I'm coming home
st64  Dec 2013
worm-wheel
st64 Dec 2013
the farewell of the magical-masque
           the dance of the whirlwind
           the twist in valediction
a pantomime of comedy dripping in life’s heat, its tragedy blooms forlorn
silently the mountain-ranges stare
the sky-face won’t relent and contemplates the open-disease in homes*


1.
disguised as simple relief – rescue lies cooing in the palm
     crumbling in blue-ash beside your grinding-palate
     you reach for pen and paper to appease an entity unknown
shrouded in grey, no scavenger can touch the head of one
who carries blessings in the scabbard – the present worthy of now

stairs are slippery, fish are mouthing, anger grows
     symbols hop along outrageous, so stylised and signs come in decisive
     all at once, almost
there is some purchase in the widening-valley
when climbing-feet need to rest on your narrow angular-will
and wait.. (before them chips rain down)
until the merry-turnstile comes in view


2.
the worm-wheel goes blank a while
and out tunes a dastard-and-devilish prank, courtesy of blunted-fate
sacred-fillies get hacked at by small silver things and they lie slaughtered on stark-plains
and the orb dips in reverse this time
a sooty-traveller from the western-flank
               glances out at massive-figures at supine-rest
               gets startled by the rude ***-fire
eyes slit and pates distort in hostile-fever
at the starling-ingénue in mock-fatigues and fake-epaulettes
but cheering up with wry-humour makes your feet
           a touch too slow to react in time
           and the halberd comes crashing down
well, the last thought you hold before your next one
is how utterly beautiful she looked at the station
long, black hair – silky-shining in your eyes and gay-dancing in the wind
when she passed you all her sweet-love from eyes so wet and smile so quiet
and selected dried-fruit in redolent-parcel
                                   a sealed pelt-skin of unmixed-whiskey
along with fresh-baked raisin-bread in cotton-cloth
                    coarse-sliced and buttered so generous
and
a semi-rusted dry-tin rattling its bounty of macaroons through that smudgy, ***** window
what sweet-victuals to keep alive . . .



man, that journey is a long one!


                             (I’M STANDING HERE        oh, you just know I am here

AND YES -- I’M WATCHING YOU                        
                                                                ­               and no use looking round now..
      YOU CANNOT SEE NOR HEAR ME  
                                                                ­               or begging a purty-release
                                                                 ­                                             
                                  oh easy, boy.. EASY!!)                                                          ­                            
                                                                ­                                             
                   ­                                          


3.
once more, the worm wriggles in microbial-distaste
and the season’s wheel comes dangerously close to being undone
IT DOES
and seconds later, cogs fly hard in every fool’s direction
and luckily.. you catch some in your face.. mouth agape
        crushing your tongue
        splintering all your dental-treasure
        smashing half your reason
no time for moaning.. or eroded-regret.. or even to feel your lips in ribbons
for, when they turn their backs, you will know
what to do..


because you’ve picked some pearls the hard-way..
that atonement could well appear in spells
of any shape
or size




not so?





S T, 30 dec 2013
beautiful in the mountains.. Jupiter enjoys the odd (but needed) breeze along with sweetness of Nature’s sounds  :)



sub-entry: ten times

you get ten times to refract your pain
mind your head now
the ceiling’s low
the parchment’s dry
and then some..

wait a little while.. it all comes round :)
Dalton Bauder  May 2013
eye
Dalton Bauder May 2013
eye
in perfect motion, lost in time,
I subdivide the outer lines.
the enigmatic parallax;
dilation of the centered mind.

microbial in grander schemes,
my breath is born to exit me,
inhale the holy entity;
become the dreams
in vivid scheme.

intrinsic shapes of destiny
envelop my entire being;
a calculated entropy
that grants subconscious unity.
magnified, this smoke will bleed
into my every living deed,
tied into every breath i breathe
I stretch my being; exude peace.

I’m only what I dream to be,
as I ask myself to pray for me.
Bassam Mar 2010
Proceeding in the wake of mankind's scourge,
Spoken are the words of this great demiurge,
At dusk the cowled of the night shall emerge,
And convey a true evil on God's Earth to resurge.  

Unleashed and unfathomed, behold the words of a phantom
Turning cities into craters and the oceans to chasms,
Imagine: a picture perfect world, can it exist,
Without the plague of the human race, lost without a trace in abyss!

Ignorance tragic, the magic of bliss,
Static damage to the rabid on this planet of ****,
An example of this: the progression of time
Deteriorating in abundance, a final judgment for mankind.

Exterminate the population, man, woman and child,
Convictions, the arrival, apocalypse nigh!
None will survive, total disaster, blood stain alabaster
Abstain, refrain, salvation from a heavenly ******* shall be sought in vain.

Unexplainable cataclysm,
The missing piece of the puzzle unseen in catechism,
But it was written somehow and somewhere
And the emergence of its purpose was unclear, deny what you fear!

The end is near, malevolent seraphim invade,
The end is here, a feeble humanity kneels and prays
It was revealed, none prepared and none spared
And act of evil, fitting for the slaughter of a people.

Mephistophelian ascension,
A requiem for the souls of the ruined be sung
For a destruction, beyond all human comprehension.
Alarum with no human intervention!

An apoplectic annihilation, fed lies by inhalation,
Microbial immolation, infected detestation,
Evasive evasion, catastrophic, melancholic
Leaving mankind intoxicated by his own narcotic

Whilst hypnotically induced, the demons invade,
Equestrian quartet lead the massive evil brigade
A battalion of stallions, on fray to slay grace
Laid to waste in the face of the inhuman race.

To keep pace, without a trace, Messiah on Fire
In dire need, erase calumny the Heavenly liar feeds
Desire breeds and hatred grows
Within those a crueler fate chose the pyre to bleed.

An ascension to an unknown throne overthrown,
A crown adorned in thorns be thy Kingdom's scorn
To the Black, I am sworn, prophet to the swarm,
The scores of the forlorn born to battle in the storms

Of Ragnarok, the magma rocks rain from the sky,
The Earth will end in fire, watch the genesis die.
Terrestrial crucifixion, the mortals' last affliction,
Desperation bringing forth a dogmatic dereliction.

Infliction of pain, deadly diction to the slain in vain,
A spoken name, confliction causing friction
An addiction to the wicked, auspicious yet pernicious,
Foreboding a sinister outcome of ecumenical wishes.
**lyrics by Samuel H. Kelly for the Rare Form "All Will Suffer!!" EP, released in 2004.
Alexander Nelson  Apr 2012
reach
Alexander Nelson Apr 2012
dont need relief from cluster headaches, hopefully i never will
i don't need pink blotting paper
i don't want anxiety to the point where I can't breathe
i don't want to rhyme anymore
i just want to understand why the man in the toll booth
annoys me to any extent
i hear something as i walk past him
maybe its his thoughts, or just the physical
presence, of him tapping the metal siding
maybe he's an introvert that's come out of hiding
maybe i just lied about not rhyming, i can't decide
i honestly can't decide anything anymore, it's beyond indecision
its bent derision of vision
it's beyond confusion, because the confused know that they are
im confused about whether im even confused in the first place
i am... urges, i am... impulse, im not...progress, or it seems that way
i could be progressing in relative terms, that's if einstein was right
but who the hell knows if he was
humans have been on earth for 5 million years, a drop
in the geological bucket, **** it
where's motivation when all collected knowledge
could "in itself" be progressing in the wrong direction
at that point we are the id and nothing more
we have nothing to offer microbial nature
on any other planets nomenclature, mars for instance
has a higher knowledge, their +1, we're -100
im just talking this system, god knows what's just 4 lys away
probably nothing, but nasa still wants to take more pictures of uranus
kiss it *****
smallblank May 2014
As you read this you are traveling 220 kilometers per second across the galaxy and I cannot stop thinking about the fact that ninety percent of the cells in your body carry their own microbial DNA and are not "you."

Which explains why your eyes likely originated from the belly of a star.

There is always a light at the end of every tunnel and if there isn't you should consider screaming until your voice echoes across the galaxies tucked within your irises.

I wonder if the trees know they must die every year for their leaves to become new again. Wounds line your heart like sticky notes left in the sun and the origin of you has been faded.

Black is the color of death but to your funeral I will wear white.

I will celebrate the death of everything trembling inside of you and stitch together funeral dresses for every version of you I watched leave without a goodbye.

I will wear white to your funeral to celebrate your rebirth soon to come.

Many hands will tie your old self to a chair and set the line between real and ideal on fire but only time can turn a flame into embers.

Most of the cells in your body are just empty space and skin is only a burial ground for old versions of yourself to die.

Your fingernails are only tiny shovels digging up a bed of dirt to plant new pieces of your DNA in.

I will cover my best dress in dirt and stain every white hem in celebration of the death of the fear inside of you and the birth of hope.
Being beloved must be pleasant
Depended upon yet carefree
What it must be like to deliver
Subtle nods, vague affirmations
Lacking regard of another’s interest
Seeing into you, from a skewed angle
Hoping to get recognition
While ostensibly unaware
Of the microbial ambivalence
Pervading the room from your eyes
Each morsel shared ‘twixt each
Whether intimate or scrupulous
Swept away with a shrug and a call
From a waiter and an ex
Like an invitation to despair

— The End —