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Àŧùl Dec 2016
Preventing contamination,
A constant challenge in cell culture.
Contamination not only affects,
The culture in question and,
Costs time and money,
But also endangers the reproducibility of results.

No cell culture problem,
Is as universal as that of culture loss
Due to contamination.

Generally, contamination may be separated,
Into categories of microbial,
And eukaryotic contamination.

Examples of microbial contamination include:
Bacteria (including Mycoplasma),
Fungi and yeast;
Eukaryotic contamination includes:
Cross-contamination with other cell lines.

Bacteria, yeast and fungi,
The three more common types of contamination,
But luckily these forms are often detectable,
Under the microscope and,
By visual cues,
Like colour or turbidity changes in the medium.

Mycoplasma is a small genus of bacteria,
That lack a cell wall and for this reason,
They remain unaffected by common antibiotics.
They are also difficult to detect,
With standard microscopes,
Due to their size, about 0.1 μm in diameter,
And the fact that they often attach to host cells.


To prevent contamination,
Use 70% ethanol for disinfecting,
Equipment & surfaces,
Related to cell culture.
Sterile filter the media first,
Before bringing to the lab.

Fetal Bovine Serum,
A potential source of contamination,
Contains mycoplasma.
Filter it at 0.1 μm, or,
Gamma irradiate it.
Aseptic technique,
Necessary.

The laboratory workers be the last,
But not the least source of contamination.
Teach them the ideal laboratory practices,
To ensure asepticity in a laboratory.
Source: American Laboratory

For revising an important topic from Animal Cell Culture.

HP Poem #1299
©Atul Kaushal
Reece Jan 2013
Brought forth from a darkness so secure, baby boy relentless in the pursuit of education gazed upon the egg shell walls and sterile environment.
Breathing as if it were natural.
A construction of steel and concrete was the new cocoon , the window was an eye to a neoteric world. Bright white lights shone from within and a dull foreboding cloud loomed beyond the glass for the child to appreciate.
Mother exhausted collapsed sighing. She is the antidote to all that is evil, she is the mother to the world. A usually stick-thin figure now distended but leisurely relaxing.
Nursing her son as if it were natural.
Swooning nurses swaddle infants, the original factory workers. Substantial days grafting, workhorses prancing throughout aseptic halls.
The heroines of our world.

A tribe appears from dust clouds, over the dunes, panting, half-alive. Heavenly Ethiope arriving in time for the world to begin. Tumescent in her ecclesiastic luminescence bearing a King destined to travel great distances primed for expulsion from the cimmerian safety of the womb.
The seas of the earth accumulate before the small band of tall-standing creatures of exquisite anthropomorphism. Creatures from across the great unexplored continent at the centre of our world gathered in frenzied crowds. The Elephants marched in earth shattering herds, the lions of the Savannah put aside their differences and sat amongst the  wild dogs of Ethiopia and the grévy's zebra, the dibatag stood and eagerly waited. Shrews, mice, gazelle, otters, cheetahs and giraffes all surrounded the tribe. Taking a silent vow and allowing stewardship to be passed along to a new generation.

Every mother is the mother of the earth. Her earth, the personal concept of earth that only she may understand.

Both children are connected by the planet they learn to walk upon. Connected by a thousand generations but connected nonetheless. They are one and the same. Each bought into a world in which they have no knowledge, each merely a slate eager to be scrawled upon by the elders of this fine rock.
Àŧùl Aug 2016
Long gone are the days of ******,
No more people say, "Alle Rufen ******".

Germany is now aseptic and really safe,
Na'zi germs are no longer there.
I have long cherished a dream to settle in Germany, the land of my paternal ancestors over 50 generations ago.

My HP Poem #1111
©Atul Kaushal
Soraya Carpenito Nov 2011
In the morgue, the aseptic light
Was flickering upon it;
The livid, bruised, black and blue
Lying body of Love.

-Honey, It's dead, you see!
-Yes, sweetheart, but how did we
Come to this?
-Pass me the lancet and
Then we'll see.

A sharp cut was made on
The right temporal lobe of the brain;
The synaptic membranes were
Damaged, the reciprocal nerve-racking
Jealousy had made the brain collapse.

A big incision was made upon
The ribs: into the lungs no more
The vital breath of Love, only water
And mud were clogging the alveoli.
Love had drowned in the sea of adultery.

The last deep cut was made upon
The heart: the still valves and
Ventricles hadn't pumped
Blood and passion for long.
So, there's nothing else to do,
My dead love!
Valora Brave Nov 2015
Precision lived in the way she spoke
Cadence like a poem
She could have wrote.

She wore heels in my kitchen
as she danced around the sink.
She had been soaking in music all day,
she needed the noise to think.

I could feel her desire and approval
of all my corners and sharp edges
and all my performances, she applauded
never seeking my reform
She just wanted to slip out of the face and clothes she had worn
All day.

But those heels stayed on
tapping the hardwood floor
I could hear her in my kitchen
smothered by the bright red walls.

But those heels stayed on
so she could make the music,
as she danced around like
there was a light flowing in.
I could feel aggression in the acoustics
that somewhere beneath all that soft skin
something learned to be muted
a streak of darkness,
that small spot she wouldn't let me in
She held it so dear and so tight
I couldn't get near

When we fell to ashes dreaming of ways to connect
I could feel the abstract effect
of her fingertips at the base of my neck
on the side of my cheek
in the curls of my hair
tangled and tugging
Little tears she left
on my back and arms colored in white
because I wanted to harness her light

I should have known she'd be gone before she left
so when I saw her there
a luminous, nonchalant stare
I knew she was simply unaware
of how my kitchen is still swollen with the music
of her clicking red heels
of how my floors have deep wounds that are beginning to peel

So, I burned through August like a pack of cigarettes
With a distaste for oval-faced, brunettes,
And I'm trapped inside the mind of a theorist
pretending your vacant pity
will make my sight clearest

Red morning commutes
awoke in September, with optimism to settle disputes,
Riding in the soft rain of yellow leaves,
but I'm not the only one who grieves
over dancing, straight-haired women
in red high heels

So when she appeared in my atmosphere
somewhere  behind dark curls, I began to feel
How afraid I was to draw you near

Her mistrust of my performances
and sharp edges
she soaked in the soft piano that drummed from the fireplace
and spilled in through the skylights in my room.
We laid in bed through Sunday's noon.
Silent kisses became the only music that played -
the rustle of sheets, quiet moans
the subtle changes in tone
in and out, constant static.
You didn't feel the need to fill the silence.
So I let the silence in.
We used to be such experts on reliance
Now we were never under each other's skin
This was not a game, either of us was going to win

I heard you come through my front door
you were all smiles in a small black dress
The lack of guilt behind,
the desire to watch your undress
was an innocent crime, but I couldn't confess.

When you wrapped your arms around me
I heard your shoes against the floor
then running down the carpets
as we drifted past my bedroom door

I never confessed
How loving you was driving towards an eastward storm
away from the blue skies growing behind me in the west.
How I tried to describe you as an art form
the kind that flows into me
but I'm an aseptic scholar
To have thought of you like poetry,
when you were a watercolor
painted in sparrow black.
How I loved you like an echo,
but you were a small whisper
that never came back.


The soft trickle of rain leaves
the little cough, as your hand weaves
Her head buried in my sheets
damaged by each day in the week
We laid in bed, wondering what wouldn't last
and waited for October to pass
Akemi Oct 2017
upon coming to the exit and birth, beginning, origin of the supermarket, I had the vision I was pushing my own body out of the morgue/into the abyss.
sleek, ultra-modern, aseptic carrion floor, processed through checkout, aisles, background fuzz, and the pointless chatter of deciding upon this or that alienated labour product.
the worthless time, the bare destitution, the surging eyes fixed across a nothingness that reduced both you and i to economic ex--
a holy verification of existence together in this ******* astronomically ******* up world.

blood at my index, slit along the serrated edge of a tin, metal scrap, upon a mountain of flesh; empire, bread and sons.
mass, *****, incarceration, brand loyalty, ethical spending, assimilation; all wallets bleed the same.
my race, my class, my gender, my age; DIY elevator pitch.

there.s nothing left.
there.s no.thing lft.
there.s n.thing .ft.
th.re.s g.f.
h.re.
e.

fine thread through the arched belly of a bleached whale, blood mixed with the grease, and salt, and death.
make me lack.
Dearth Feb 2019
Your aseptic words
Carefully crafted
Like a careful carpenter
Or a surgeon with his scapel
Wreathes me with fire and smoke
Sophistry in its finest form
PABRO Oct 2021
Like a dreamer,
Was happy to lay-down up the galaxy
Just as an astronaut ,
I kissed the moon's feet like a missionary.

Before bed,
aseptic of flute, violin was as a dose.
And pink lips sips and stamps the forehead
Beside me was a night rose

It was a great night,
To ignite.
The Might night of light in us.
Like a righteous.
Jun Lit Oct 2021
Hope was delivered quickly, mercifully,
as the aseptic needle silently, expertly
pierced the anxious skin of my upper arm
bared to its untattooed, obese reality
and scarred deeply with forgotten badges
from islands and mountains and forests
and caves, with souvenirs and tokens
from clingy rattans, unforgiving wasps,
solicitous leeches, and hyperactive biting midges.

Pushed by magmatic desperation, something
imposed by elected incompetence, fudged
as a destiny of an unfortunate nation,
I toed the line of the long queue, hiding
my rhinitis-ruled nostrils and mustached
mouth from the many dreaded arms
of SARS-CoV-2, uneasily shielding
my embarrassed face from sneezed aerosols.

Aging paranoias of undignifiedly drowning
in one’s own phlegm unconsciously fuel
the tired and greying servant. Respite is not
as appeals for help to ease the burdens
of mountains of debt, and so sadly, yet
the beloved, alone, succumbs to death.

We’re all hostages - and the ransoms demanded
by this protein-coated tyrant are costly,
unjustly. Incarcerated by our fears of being
caught within the nets of this pirate at the sea
of our existence, we are, I am, grasping at all
but the last strands of a rotting rope – hope,
diminishing, flickering hope of salvation
from pathogenic damnation. Come messiah!

Likened to Christmas Stars shining bright,
the sages of Science illuminated our dark night
And through the ***** of a hypodermic needle,
Hope was delivered quickly,
mercifully,
compassionately . . .
This was written immediately after the author got his second dose of AstraZeneca. It was read by the author himself as a contribution to the Virtual Cultural Concert (VCC) held on 09 October 2021. The virtual cultural event was organized by the UPLB Office of Alumni Affairs, and the Classes of 1971 and 1981 in celebration of the 103rd UPLB Loyalty Day (10 October 2021) with the theme “Bigkisang UPLB at Alumni para sa Matagumpay na Pagbangon Mula sa Pandemya.” [Strong Bonds between UPLB and Alumni Toward Victory in Recovering from the Pandemic]. The poem is dedicated to all UPLB Alumni, especially those in the Sciences, Medicine, and allied fields in the frontlines.  In Part, the poem is also a thanksgiving to Science & Scientists.

https://www.youtube.com/watch?v=0aQv-ZpRqyY
ohNoe Jul 2020
La Vida En La Villa Strangiato


Wondering what remains inside this aged shell...
  are there any poems still wanting me to tell?
will the words still write themselves
  when I whisper sweetly inside myself?

Even as broken as i remain
  shuddering within this shattered domain
are there star stories still in these skies
  cuz I still see them in my inner eyes

Music is terrifying
  touches far too deeply and truly
My muse no longer clarifying
  i'm left bereft and too unruly

death hath touched me too many times recently
  continuing to steal amazing beings from me
and his evil cousin cupid
  keeps ****** me almost as hard

that verse was all about meaning
  with zero respect for rhyme
the thing is,
oh aseptic poem ****...
*******

blech blah meh in my maw
  hate the taste of pain this raw
days weeks months years ago
  none of it has the decency to just go

i'm tired
  inside
never been this tired
  even when hope first died

believe it or not
  i still laugh a lot
but it's a momentary meeting,
  ephemerally fleeting

why is limbo a frozen inferno
  how why does it burn so badly
and why does it ******
  at my emotional chasm

almost everything that's me is amiss
  mostly unable to miss this abyss
the one void i can't avoid
  the tattoo inside i can't hide

suppose i should be glad to still feel
  but i sure would like to finally ******* heal  
is what I want from and for me merely in purgatory
  or is my end game destiny an eternal empty

cuz, you noe, like i always used to say sometimes,

— The End —