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Àŧùl Dec 2016
We count cells by manual methods,
Using the counting chamber,
Plating & colony forming unit count.

We let them be counted automatedly,
Using electrical resistance,
Flow cytometry & image analysis.

Then there is this indirect method too,
Using spectrophotometry we count,
Or even by the impedance microbiology.
Cell counting methods used in Animal Cell Culture include the above three main categories and then seven sub-categories are divided among the three chief categories.

There are two manual counting methods:
a. using the counting chamber for counting each one individually, and
b. plating and CFU (Colony Forming Unit) count.

Three automated counting methods are there:
a. using electrical resistance,
b. flow cytometry, and c. image analysis.

Two indirect counting methods are there too:
a. using a spectrophotometer, or
b. count by impedance microbiology

HP Poem #1334
©Atul Kaushal
Daron Bigby May 2015
This life didn't come with a manual
We're forced to manually go through its ups and downs
Getting spun around on society's notion of how to live
You see, society works like a model T factory
Trying to put us down a conveyor belt
Place us in a mold and push us out like that's really how we're supposed to be
They told me I need to graduate high school at 18 Finish college at 22
Then go to work wearing a tie in a cubicle
They told me I need to provide for a wife and two kids
Bring home the bread in the form of 5-6 figures
But here's what they didn't tell me
They didn't tell me what to do when college tuition was raised again
I mean I'm already eating three square meals of ramen noodles just to make the payments
They didn't tell me that the one class I need to graduate is no longer offered
So I came all this way just pick another major
They also didn't tell me that they only hire people with experience
Now I'm stuck with a piece of paper and mountain of debt
And it's one of the best kept secrets that society tried to hide the horror
That I paid 100 grand to say can I take your order
They also didn't tell me that it's hard to find my queen
In the sea of self-entitled princesses that only want my money
They want relations, they don't want relationships
They crave the attention but none of the commitment that comes with it
Society is so focused on creating a perfect standard of living
That they forgot to tell me what to do when it perfectly unravels in front of me
And they continue to push people out of the factory
While I'm swimming in the byproduct they conveniently left me
This life didn't come with a manual
So society can't fool me by creating rules on how to live
Because racial divides say we stereotypically live differently
Yet they continually expect us to live equally
I dared to be different and chose to live for me
I was sick of living vicariously through the rules of society
And decided I am the pilot to my own destination
Flying to my own creation of life
After all, this life didn't come with a manual
Amitav Radiance Jun 2014
Each one of us writes an autobiography
Pages composed with anecdotes and memories
Pen dipped in the ink from our soul
Helsy Flores May 2014
If you wanna be a part,
There really is no art.
Be funny, handsome, and smart,
And you have my heart.
2013

— The End —