We use ETBR in the laboratory,
Ethidium Bromide is a poisonous dye,
And it is to be used carefully,
RedSafe is an even deadlier alternative.

Give special attention to its use,
Low - very low amount will do,
Or it can cause health problems,
Victory over nature can be constructive,
Exposure to it can cause cancer,
Should our efforts help in medicine.

Also used is an alternative marker dye,
Lacuna not entertained in it either,
Wear gloves always in the laboratory,
Always in this field of proteomics,
Youth may be affected otherwise,
S**hall be always keeping myself protected.

Come on buffalo,
Open your mouth,
Of your oral cavity,
Let us collect some tissue,
And let us collect some saliva too,
And then we test for some trefoils,
Fingers crossed – let the expression be true.

It has got to be there,
We know it for humans,
But of buffaloes, we know not,
Let us perform a preliminary study,
There has not been much research,
There is just a foggy, hazy oversight,
Scientific charm – the expression is positive.

Molecular markers in the electrophoresis unit,
Mixed with a visualising dye – the ETBR,
Yes, they will dance positively as expressed,
Against 400 base pairs expressed are the TFFs,
Tough to master this technique moderately is,
We have to take numerous precautions,
Especially with the poisonous visualising dye.