They Call It Heresy,
We Call It Genuine Science

We designed the genes' primers,
Ordered them along the oligomers.
Our aim is an elaborate one,
It involves molecular cloning,
Sequence characterization, and
Relative expression analysis of
Bovine Trefoil Factors.

Now we hope to clone the gene,
The gene which is of a bovine origin,
By extensive working hours input,
And bearing in mind the risks,
Of not getting the desired output,
The possibility of failure always therein,
But pregnancy, healing & immunity it's governing.

Three types of trefoil factors there are,
TFF1: It suppresses gastric carcinoma,
And also helps in pregnancy,
TFF2: Helps exclusively in cancer research,
TFF3: Helps exclusively in pregnancy maintenance,
And also our prime interest.

After cloning the genes,
We have to sequence them,
And after characterization,
We have to analyse them,
After relative expression.