Resuspension
Centrifuge & resuspend the oligos,
The precursor to your macromolecule,
Follow it by concentration & dilution.
To avoid resuspension difficulties,
Heat the oligos to 55º C, and,
Vortex in between thoroughly.
Storage
Optimal conditions,
For standard DNA oligonucleotides,
They be followed closely.
Store them at –20º C for long,
At 5º C while performing procedures.
Also, store them with fluorophores,
For better visualization later.
For standard RNA oligonucleotides,
The conditions be more stringent.
Feb 9, 2017
Feb 9, 2017 at 2:40 AM UTC
Resuspension
Centrifuge & resuspend the oligos,
The precursor to your macromolecule,
Follow it by concentration & dilution.
To avoid resuspension difficulties,
Heat the oligos to 55º C, and,
Vortex in between thoroughly.
Storage
Optimal conditions,
For standard DNA oligonucleotides,
They be followed closely.
Store them at –20º C for long,
At 5º C while performing procedures.
Also, store them with fluorophores,
For better visualization later.
For standard RNA oligonucleotides,
The conditions be more stringent.
My HP Poem #1428
©Atul Kaushal
