#procedural
Resuspension
Centrifuge & resuspend the oligos,
The precursor to your macromolecule,
Follow it by concentration & dilution.
To avoid resuspension difficulties,
Heat the oligos to 55º C, and,
Vortex in between thoroughly.
Storage
Optimal conditions,
For standard DNA oligonucleotides,
They be followed closely.
Store them at –20º C for long,
At 5º C while performing procedures.
Also, store them with fluorophores,
For better visualization later.
For standard RNA oligonucleotides,
The conditions be more stringent.
Feb 9, 2017
Feb 9, 2017 at 2:40 AM UTC
I used to write just to read on and on
In a listing manner, with a scrolling eye
But no more do I
Write in such a manner as this
There’s no desire to be found
As those days have long since passed me by
And now I only write for myself, the ID
As compared to the length which these pages provide
And I stop flowing waters
Dead in their tracks, without so much as a whisper
Because I’m no longer afraid to speak
Or to be seen here within these imperfect lines
May 20, 2019
May 20, 2019 at 9:50 AM UTC