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Àŧùl Dec 2016
Preventing contamination,
A constant challenge in cell culture.
Contamination not only affects,
The culture in question and,
Costs time and money,
But also endangers the reproducibility of results.

No cell culture problem,
Is as universal as that of culture loss
Due to contamination.

Generally, contamination may be separated,
Into categories of microbial,
And eukaryotic contamination.

Examples of microbial contamination include:
Bacteria (including Mycoplasma),
Fungi and yeast;
Eukaryotic contamination includes:
Cross-contamination with other cell lines.

Bacteria, yeast and fungi,
The three more common types of contamination,
But luckily these forms are often detectable,
Under the microscope and,
By visual cues,
Like colour or turbidity changes in the medium.

Mycoplasma is a small genus of bacteria,
That lack a cell wall and for this reason,
They remain unaffected by common antibiotics.
They are also difficult to detect,
With standard microscopes,
Due to their size, about 0.1 μm in diameter,
And the fact that they often attach to host cells.


To prevent contamination,
Use 70% ethanol for disinfecting,
Equipment & surfaces,
Related to cell culture.
Sterile filter the media first,
Before bringing to the lab.

Fetal Bovine Serum,
A potential source of contamination,
Contains mycoplasma.
Filter it at 0.1 μm, or,
Gamma irradiate it.
Aseptic technique,
Necessary.

The laboratory workers be the last,
But not the least source of contamination.
Teach them the ideal laboratory practices,
To ensure asepticity in a laboratory.
Source: American Laboratory

For revising an important topic from Animal Cell Culture.

HP Poem #1299
©Atul Kaushal
Charlie Rose Dec 2020
I grew up on heroes old and new
Thinking there was no wrong they could do
But get rid of rose glasses and they're the worst
And I woke up to a clergy that turned. out to be cursed
Now I can't trust people I see
On the internet or TV
Because half of my childhood celebrities
Turned out to be pedos, grifters, and Nazis

Some times you have to let your heroes die
Give a wave and a nod goodbye
Send them off with a ******* up
And hope next time you have better luck

Can't trust the storytellers that made your fantasies
They want you and queers like you deceased
Can't play pretend in a superheroes game
When all the directors fund your personal bane
I wouldn't trust the beliefs that raised me
Because I've grown old enough to peek and see
That its foundations are corrupted by empire
One more genocide and I'll set the **** pulpit on fire

Some times you have to let your heroes die
Give a wave and a nod goodbye
Push them away from who you see
And rethink all those memories

I once trusted some political heads
Thinking they had good interests in their stead
But red or blue they all bomb and starve and censor
Now I have to try to get my own beliefs and center
I blindly trusted the medical process
Until I saw prejudice and the reproducibility crisis
I blindly followed the worship of success and riches
Until I saw the dead that world left in the ditches

Some times you have to let your heroes die
Give a wave and a nod goodbye
Shoot them yourself if that's what you need
Because how else can you move on and succeed

History was taught to me in one way
Every account matching up to the day
But I looked back and saw a library
Of stories the classroom never did see
The people who raised me said they were saints
And that's the picture their friends all paint
But get home at night and you'll find out
What the kids don't know to talk about

Some times you have to let your heroes die
Give a wave and a nod goodbye
Never look back for a second glance
Don't give the ******* another chance

Some times you have to let your heroes die
Give a wave and a nod goodbye
Because if power corrupts and they're infested
Don't reminisce long enough to get invested

Some times you have to let your heroes die
Give a wave and a nod goodbye
Throw down your celebrities and your idols
Be your own hero and disregard titles
Kuzhur Wilson Aug 2014
Intelligent believers of democracy,
Let me inform you with a great surge of emotion that I am a candidate in this election
I beg you, request you, beseech you to make us win with great majority  by casting every vote of yours for our symbol

I don’t have to recount the great services rendered by our logo in houses, by-lanes, churches, temples, offices, hotels-  why, in buses, hospitals, monasteries, cemeteries, and every nook and corner of the land

About its great desire to fill even the stomachs of those little children who sleep along the roadside, with no one to look after them

Our sign cannot ignore the mothers and sisters who work in factories of sighs, with only half their stomachs full. That’s why even after being totally spent, it resurrects itself again and again.

Its social sense which decries that even those bodies  on hospital beds, half-burnt, should get justice.

Wont the dead have unquenched desires
Just like the living?

The greatness of our emblem and its universality which embraces unborn babies, the living and the dead, without any consideration of caste or creed or ***

About its reproducibility, the sense with which it can raise or lower itself as the opportunity demanded, its will power which helps it work with a passion, its power to please, its divine gift to give peace and happiness

What about its readiness to sacrifice even the last drop? It thinks only about giving! Please do not fall into the traps of the other signs which are never satisfied whatever it got, and which are ready to split any moment.

Let me ask  you, have we come first in anything? China is standing like its great wall..let me remind you that  if everyone tried together to raise our symbol to great heights, we can at least come first in population

Please do not let go of the chance to win, listing unpolitical arguments like headache, hunger, hatred etc

Our slogan
Contentment for everyone from children to old people

A land where milk flows
translation : Anitha Varma

— The End —