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Growing up on a farm
I lost count of
The hundreds of times
We rotted compost
And scooped manure
In order to make things grow.

I figure that's the reason
I still believe in my life
Blooming.
(I)

Pale mulberry was the sky,
No bird dared to fly!
Thus all seemed wrong,
But then, you came along
Suddenly like summer rain
And quelled away my pain.

(II)

Velvet blue was the sky,
No bird dared not to fly!
Thus all seemed right,
And as pure as a cloud in white,
When suddenly like the rainbow,
You quelled away thy heavenly glow.

(III)

Dark grey is the sky,
No bird seems to ever fly!
Athwart my wild blue yonder
Where I, indignantly do ponder
Night and day wondering why,
We can't give it just one more try.

(IV)

Pitch black is always the sky,
But, faster than any bird I'll fly!
Swifter than a scudding cloud
Whilst calling upon you so loud,
All the way to a strange plain,
Just to ever feast about you again.

(V)

Magenta magic will always be the sky,
When once again we'll merilly fly!
Then, flowers once again shall bloom,
To see you and me as bride and groom
By a placid Mulberry Moon on the rise,
To kindle our enchanted paradise.



©Kikodinho Alexandros
Jumeira, Dubai
1st December 2016
***!!! Can't really believe it that among the myriads upon myriads of beautiful poems here at HP, this poem has turned up the daily. Thank you so much dear friends to have catapulted me to stardom for the second time...I'm really all gratitude.

#Retrospection
#Nostaligia
#Lonesome
#Craving
#Wishing
Words

We live in a wired and weird world
where meanings of our words
are paper-thin tissue and torn
tarnished and worn by wear and War.

© M.L.Emmett
 Dec 2016 brian odongo
Nessa dieR
In your arms I found the open ocean;
Tides, waves, my serene sea,
The most pleasant feeling of a morning breeze.
In your eyes the best night skies;
A Rhythm bright enough to leave the sun behind
and wake up the night in just a heartbeat.
My heartbeats.
Cracked and Irregular by your every move.
In your fingers untold mysteries;
tangled within my own in secrets with the promises of never letting go.
In your hair my favorite melody;
Loud, and ruthless music for my deaf ears:
A Symphony only I can hear.
In your lips my muse;
Better than Erato and Calliope combined,
Carelessly whispering verses
To last me the entire day,
Softer than the birth of Roses.
My Roses.
For they are just for me,
Sprouting from your lips, and blossoming with my touch.
Our touch.
*In you I found poetry.

With verses resting at your lips,
My muse
Ma
Ay ma
I just don't get it
Always about them ma
They ain't never asked you huh,
Never asked you ma,
Never cared to see
What you had to tell us
About this white mans world
How we are afraid
They **** us ma,
They don't believe us,
Or they probably don't care
I saw my man get beat ma,
Right down the street
By the corner store
Had his hands up ma,
He was crying, afraid of dying
Ma, he was so scared
And if they don't **** us
Then they lock us ma,
For nothing, just like dad.
But they don't care ma,
Same old story.
They ain't never asked
They ain't never cared to see
Whats its been like for you and me.
Here you and I stand
Beneath the cosmic graveyard
Engulfed in twillight
 Dec 2016 brian odongo
Àŧùl
Preventing contamination,
A constant challenge in cell culture.
Contamination not only affects,
The culture in question and,
Costs time and money,
But also endangers the reproducibility of results.

No cell culture problem,
Is as universal as that of culture loss
Due to contamination.

Generally, contamination may be separated,
Into categories of microbial,
And eukaryotic contamination.

Examples of microbial contamination include:
Bacteria (including Mycoplasma),
Fungi and yeast;
Eukaryotic contamination includes:
Cross-contamination with other cell lines.

Bacteria, yeast and fungi,
The three more common types of contamination,
But luckily these forms are often detectable,
Under the microscope and,
By visual cues,
Like colour or turbidity changes in the medium.

Mycoplasma is a small genus of bacteria,
That lack a cell wall and for this reason,
They remain unaffected by common antibiotics.
They are also difficult to detect,
With standard microscopes,
Due to their size, about 0.1 μm in diameter,
And the fact that they often attach to host cells.


To prevent contamination,
Use 70% ethanol for disinfecting,
Equipment & surfaces,
Related to cell culture.
Sterile filter the media first,
Before bringing to the lab.

Fetal Bovine Serum,
A potential source of contamination,
Contains mycoplasma.
Filter it at 0.1 μm, or,
Gamma irradiate it.
Aseptic technique,
Necessary.

The laboratory workers be the last,
But not the least source of contamination.
Teach them the ideal laboratory practices,
To ensure asepticity in a laboratory.
Source: American Laboratory

For revising an important topic from Animal Cell Culture.

HP Poem #1299
©Atul Kaushal
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